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a2-M clia kit :: Human alpha2-M (Alpha-2 Macroglobulin) CLIA Kit

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Catalog # MBS2530884
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

alpha2-M (Alpha-2 Macroglobulin), CLIA Kit

Full Product Name

Human alpha2-M (Alpha-2 Macroglobulin) CLIA Kit

Product Synonym Names
alpha2M; a2M; Human a2-M (Alpha-2 Macroglobulin) CLIA Kit; Human a2-M
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
103950
Species Reactivity
Specificity
This kit recognizes natural and recombinant alpha2-M. No significant cross-reactivity or interference between alpha2-M and analogues was observed.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of a2-M clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
a2-M clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of alpha2-M concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to alpha2-M. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for alpha2-M and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain alpha2-M, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of alpha2-M. You can calculate the concentration of alpha2-M in the samples by comparing the RLU value of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of a2-M clia kit
a2-M clia kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2530884. Click to request current manual
NCBI/Uniprot data below describe general gene information for a2-M. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
163,291 Da
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
NCBI Official Synonym Symbols
A2MD; CPAMD5; FWP007; S863-7
  [Similar Products]
NCBI Protein Information
alpha-2-macroglobulin
UniProt Protein Name
Alpha-2-macroglobulin
UniProt Synonym Protein Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt Gene Name
UniProt Synonym Gene Names
CPAMD5; Alpha-2-M  [Similar Products]
UniProt Entry Name
A2MG_HUMAN
NCBI Summary for a2-M
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
UniProt Comments for a2-M
A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular region

Molecular Function: calcium-dependent protein binding; enzyme binding; growth factor binding; interleukin-1 binding; interleukin-8 binding; protease binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity; tumor necrosis factor binding

Biological Process: blood coagulation; blood coagulation, intrinsic pathway; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of complement activation, lectin pathway; platelet activation; platelet degranulation; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; stem cell differentiation

Disease: Alpha-2-macroglobulin Deficiency; Alzheimer Disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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