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A2M elisa kit :: Alpha-2 Macroglobulin ELISA Kit

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Catalog # MBS564101
Unit / Price
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  1x96 Wells  /  $480 +1 FREE 8GB USB
A2M elisa kit
Product Name

Alpha-2 Macroglobulin (A2M), ELISA Kit

Popular Item
Full Product Name

Rat Alpha 2-Macroglobulin ELISA Kit

Product Synonym Names
Rat Alpha-2 Macroglobulin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 12; NC_000012.11 (9220304..9268558, complement). Location: 12p13.31
OMIM
103950
3D Structure
ModBase 3D Structure for P01023
Host
Rat
Samples
Immunoperoxidase Assay for Determination of Alpha 2-Macroglobulin in Rat Samples
Assay Type
Sandwich
Intended Uses
The Alpha 2-Macroglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in biological samples of rats.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of A2M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for A2Mpurchase
MBS564101 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rat Alpha-2 Macroglobulin (A2M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing A2M. The ELISA analytical biochemical technique of the MBS564101 kit is based on A2M antibody-A2M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A2M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A2M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
A2M elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 2-Macroglobulin present in samples reacts with the anti-Alpha 2-Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A2M in the test sample. The quantity of A2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Alpha 2-Macroglobulin (A2M) is a major protease inhibitor in serum and an acute phase protein which increases significantly in concentration in the rat as a result of inflammation. The major pathophysiological role for rat alpha 2-macroglobulin has yet to be conclusively defined. This kit is specific to the alpha 2- macroglobulin and will not cross react with the closely related alpha 1-macroglobulin.
Product Categories/Family for A2M elisa kit
Sample Manual Insert of MBS564101. Click to request current manual
NCBI/Uniprot data below describe general gene information for A2M. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
163,291 Da[Similar Products]
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
NCBI Official Synonym Symbols
A2MD; CPAMD5; FWP007; S863-7; DKFZp779B086
  [Similar Products]
NCBI Protein Information
alpha-2-macroglobulin; alpha-2-M; OTTHUMP00000196829; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt Protein Name
Alpha-2-macroglobulin
UniProt Synonym Protein Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CPAMD5  [Similar Products]
UniProt Entry Name
A2MG_HUMAN
NCBI Summary for A2M
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq]
UniProt Comments for A2M
A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-1 binding; interleukin-8 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; extracellular matrix disassembly; platelet activation; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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