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A3GALT2 sirna

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Catalog # MBS8228204
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
A3GALT2 sirna
Product Name

A3GALT2, siRNA

Popular Item
Full Product Name

A3GALT2 siRNA (Rat)

Product Synonym Names
Alpha-1.3-galactosyltransferase 2; A3galt2; Isoglobotriaosylceramide synthase; iGb3 synthase; iGb3S
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A0A4Z3
Host
Synthetic
Species Reactivity
Rat
Specificity
A3GALT2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat A3GALT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of A3GALT2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
A3GALT2 sirna
siRNA to inhibit A3GALT2 expression using RNA interference
Applications Tested/Suitable for A3GALT2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for A3GALT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,548 Da
NCBI Official Full Name
alpha-1,3-galactosyltransferase 2
NCBI Official Synonym Full Names
alpha 1,3-galactosyltransferase 2
NCBI Official Symbol
A3galt2  [Similar Products]
NCBI Protein Information
alpha-1,3-galactosyltransferase 2
UniProt Protein Name
Alpha-1,3-galactosyltransferase 2
UniProt Synonym Protein Names
Isoglobotriaosylceramide synthase; iGb3 synthase; iGb3S
UniProt Gene Name
A3galt2  [Similar Products]
UniProt Synonym Gene Names
A3galt2; iGb3 synthase; iGb3S  [Similar Products]
UniProt Entry Name
A3LT2_RAT
NCBI Summary for A3GALT2
UDP-galactose: beta-d-galactosyl-1,4-glucosylceramide alpha-1, 3-galactosyltransferase; involved in the synthesis of the isoglobo-series of glycosphingolipids [RGD, Feb 2006]
UniProt Comments for A3GALT2
Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Diseases associated with A3GALT2 sirna
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with A3GALT2 and Necrosis
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