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A4GALT elisa kit :: Human alpha 1, 4-galactosyltransferase ELISA Kit

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Catalog # MBS901858
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Typical Testing Data/Standard Curve (for reference only)
Product Name

alpha 1, 4-galactosyltransferase (A4GALT), ELISA Kit

Popular Item
Full Product Name

Human Lactosylceramide 4-alpha-galactosyltransferase, A4GALT ELISA Kit

Product Synonym Names
Human Lactosylceramide 4-alpha-galactosyltransferase (A4GALT) ELISA kit; A14GALT; A4GALT1; P1; PK; CD77 synthase; GB3 synthase; OTTHUMP00000198433; OTTHUMP00000198434; P blood group (P one antigen) ; P (k) antigen synthase; P1/Pk synthase; UDP-galactose:beta-D-galactosyl-beta1-R; alpha 1; 4-galactosyltransferase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607922
3D Structure
ModBase 3D Structure for Q9NPC4
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human A4GALT. No significant cross-reactivity or interference between human A4GALT and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml -1600 pg/ml.
Sensitivity
The minimum detectable dose of human A4GALT is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of A4GALT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for A4GALTpurchase
MBS901858 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha 1, 4-galactosyltransferase (A4GALT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing A4GALT. The ELISA analytical biochemical technique of the MBS901858 kit is based on A4GALT antibody-A4GALT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A4GALT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A4GALT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
A4GALT elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for A4GALT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any A4GALT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A4GALT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A4GALT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of A4GALT elisa kit
A4GALT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS901858. Click to request current manual
NCBI/Uniprot data below describe general gene information for A4GALT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,499 Da
NCBI Official Full Name
lactosylceramide 4-alpha-galactosyltransferase
NCBI Official Synonym Full Names
alpha 1,4-galactosyltransferase
NCBI Official Symbol
A4GALT  [Similar Products]
NCBI Official Synonym Symbols
P1; PK; Gb3S; P(k); P1PK; A14GALT; A4GALT1
  [Similar Products]
NCBI Protein Information
lactosylceramide 4-alpha-galactosyltransferase; GB3 synthase; alpha4Gal-T1; CD77 synthase; P1/Pk synthase; P(k) antigen synthase; P blood group (P one antigen); globotriaosylceramide synthase; alpha-1,4-N-acetylglucosaminyltransferase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
UniProt Protein Name
Lactosylceramide 4-alpha-galactosyltransferase
UniProt Synonym Protein Names
Alpha-1,4-N-acetylglucosaminyltransferase; Alpha-1,4-galactosyltransferase; Alpha4Gal-T1; CD77 synthase; Globotriaosylceramide synthase; Gb3 synthase; P1/Pk synthase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
UniProt Gene Name
A4GALT  [Similar Products]
UniProt Synonym Gene Names
A14GALT; A4GALT1; Gb3 synthase  [Similar Products]
UniProt Entry Name
A4GAT_HUMAN
NCBI Summary for A4GALT
The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. [provided by RefSeq, Jul 2008]
UniProt Comments for A4GALT
A4GALT: Necessary for the biosynthesis of the Pk antigen of blood histogroup P. Catalyzes the transfer of galactose to lactosylceramide and galactosylceramide. Necessary for the synthesis of the receptor for bacterial verotoxins. Belongs to the glycosyltransferase 32 family.

Protein type: EC 2.4.1.228; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: membrane; integral to Golgi membrane

Molecular Function: toxin binding; galactosyltransferase activity; lactosylceramide 4-alpha-galactosyltransferase activity

Biological Process: globoside biosynthetic process; glycosphingolipid biosynthetic process; plasma membrane organization and biogenesis; protein amino acid glycosylation

Disease: Blood Group, P1pk System
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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