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A4GALT sirna

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Catalog # MBS8222154
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
A4GALT sirna
Product Name

A4GALT, siRNA

Popular Item
Also Known As

A4GALT siRNA (Human)

Product Synonym Names
A14GALT; A4GALT1; Lactosylceramide 4-alpha-galactosyltransferase; Alpha-1.4-N-acetylglucosaminyltransferase; Alpha-1.4-galactosyltransferase; Alpha4Gal-T1; CD77 synthase; Globotriaosylceramide synthase; Gb3 synthase; P1/Pk synthase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
353
OMIM
111400
3D Structure
ModBase 3D Structure for Q9NPC4
Host
Synthetic
Species Reactivity
Human
Specificity
A4GALT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human A4GALT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of A4GALT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
A4GALT sirna
siRNA to inhibit A4GALT expression using RNA interference
Applications Tested/Suitable for A4GALT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for A4GALT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,499 Da
NCBI Official Full Name
lactosylceramide 4-alpha-galactosyltransferase
NCBI Official Synonym Full Names
alpha 1,4-galactosyltransferase
NCBI Official Symbol
A4GALT  [Similar Products]
NCBI Official Synonym Symbols
P1; PK; Gb3S; P(k); P1PK; A14GALT; A4GALT1
  [Similar Products]
NCBI Protein Information
lactosylceramide 4-alpha-galactosyltransferase
UniProt Protein Name
Lactosylceramide 4-alpha-galactosyltransferase
UniProt Synonym Protein Names
Alpha-1,4-N-acetylglucosaminyltransferase; Alpha-1,4-galactosyltransferase; Alpha4Gal-T1; CD77 synthase; Globotriaosylceramide synthase; Gb3 synthase; P1/Pk synthase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
UniProt Gene Name
A4GALT  [Similar Products]
UniProt Synonym Gene Names
A14GALT; A4GALT1; Gb3 synthase  [Similar Products]
UniProt Entry Name
A4GAT_HUMAN
NCBI Summary for A4GALT
The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. [provided by RefSeq, Jul 2008]
UniProt Comments for A4GALT
A4GALT: Necessary for the biosynthesis of the Pk antigen of blood histogroup P. Catalyzes the transfer of galactose to lactosylceramide and galactosylceramide. Necessary for the synthesis of the receptor for bacterial verotoxins. Belongs to the glycosyltransferase 32 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - globo series; EC 2.4.1.228; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: membrane; integral to Golgi membrane

Molecular Function: toxin binding; galactosyltransferase activity; lactosylceramide 4-alpha-galactosyltransferase activity

Biological Process: globoside biosynthetic process; glycosphingolipid biosynthetic process; plasma membrane organization and biogenesis; protein amino acid glycosylation

Disease: Blood Group, P1pk System
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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