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A4GNT sirna

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Catalog # MBS828787
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
A4GNT sirna
Product Name

A4GNT, siRNA

Popular Item
Full Product Name

A4GNT siRNA (Human)

Product Synonym Names
Alpha-1.4-N-acetylglucosaminyltransferase; Alpha4GnT
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UNA3
Host
Synthetic
Species Reactivity
Human
Specificity
A4GNT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human A4GNT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of A4GNT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
A4GNT sirna
siRNA to inhibit A4GNT expression using RNA interference
Applications Tested/Suitable for A4GNT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for A4GNT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,497 Da
NCBI Official Full Name
alpha-1,4-N-acetylglucosaminyltransferase
NCBI Official Synonym Full Names
alpha-1,4-N-acetylglucosaminyltransferase
NCBI Official Symbol
A4GNT  [Similar Products]
NCBI Official Synonym Symbols
alpha4GnT
  [Similar Products]
NCBI Protein Information
alpha-1,4-N-acetylglucosaminyltransferase
UniProt Protein Name
Alpha-1,4-N-acetylglucosaminyltransferase
UniProt Gene Name
A4GNT  [Similar Products]
UniProt Synonym Gene Names
Alpha4GnT  [Similar Products]
UniProt Entry Name
A4GCT_HUMAN
NCBI Summary for A4GNT
This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008]
UniProt Comments for A4GNT
A4GNT: Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O- glycans. Belongs to the glycosyltransferase 32 family.

Protein type: EC 2.4.1.-; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; glycoprotein biosynthetic process; carbohydrate metabolic process; negative regulation of epithelial cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with A4GNT sirna
 Reference Product  PubMed Publications
 MUC6 sirna  >2 publications with A4GNT and MUC6
Organs/Tissues associated with A4GNT sirna
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >3 publications with A4GNT and Stomach
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