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AADAT sirna

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Catalog # MBS8228798
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
AADAT sirna
Product Name

AADAT, siRNA

Popular Item
Full Product Name

AADAT siRNA (Rat)

Product Synonym Names
KAT2; Kynurenine/alpha-aminoadipate aminotransferase mitochondrial; KAT/AadAT; 2-aminoadipate aminotransferase; 2-aminoadipate transaminase; Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2; Kynurenine--oxoglutarate transaminase II
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q64602
Host
Synthetic
Species Reactivity
Rat
Specificity
AADAT siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AADAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AADAT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AADAT sirna
siRNA to inhibit AADAT expression using RNA interference
Applications Tested/Suitable for AADAT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AADAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
47,784 Da
NCBI Official Full Name
kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
aminoadipate aminotransferase
NCBI Official Symbol
Aadat  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
UniProt Protein Name
Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
UniProt Synonym Protein Names
2-aminoadipate aminotransferase; 2-aminoadipate transaminase (EC:2.6.1.39); Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2 (EC:2.6.1.7); Kynurenine--oxoglutarate transaminase II
UniProt Gene Name
Aadat  [Similar Products]
UniProt Synonym Gene Names
Kat2; KAT/AadAT; AadAT  [Similar Products]
UniProt Entry Name
AADAT_RAT
NCBI Summary for AADAT
endogenous modulator of glutamatergic neurotransmission with kynurenine aminotransferase (KAT) activity [RGD, Feb 2006]
UniProt Comments for AADAT
AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Transferase; EC 2.6.1.7; Mitochondrial; EC 2.6.1.39; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - lysine biosynthesis

Cellular Component: mitochondrion

Molecular Function: protein homodimerization activity; kynurenine-oxoglutarate transaminase activity; transaminase activity; 2-aminoadipate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; tryptophan catabolic process to kynurenine; L-lysine catabolic process to acetyl-CoA via saccharopine; biosynthetic process; 2-oxoglutarate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with AADAT sirna
 Reference Product  PubMed Publications
 Kynu sirna  >1 publications with AADAT and Kynu
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