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AANAT sirna

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Catalog # MBS8216128
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
AANAT sirna
Product Name

AANAT, siRNA

Popular Item
Full Product Name

AANAT siRNA (Human)

Product Synonym Names
SNAT; Serotonin N-acetyltransferase; Serotonin acetylase; Aralkylamine N-acetyltransferase; AA-NAT
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600950
3D Structure
ModBase 3D Structure for Q16613
Host
Synthetic
Species Reactivity
Human
Specificity
AANAT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AANAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AANAT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AANAT sirna
siRNA to inhibit AANAT expression using RNA interference
Applications Tested/Suitable for AANAT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AANAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,432 Da
NCBI Official Full Name
serotonin N-acetyltransferase isoform 2
NCBI Official Synonym Full Names
aralkylamine N-acetyltransferase
NCBI Official Symbol
AANAT  [Similar Products]
NCBI Official Synonym Symbols
DSPS; SNAT
  [Similar Products]
NCBI Protein Information
serotonin N-acetyltransferase
UniProt Protein Name
Serotonin N-acetyltransferase
UniProt Synonym Protein Names
Aralkylamine N-acetyltransferase; AA-NAT
UniProt Gene Name
AANAT  [Similar Products]
UniProt Synonym Gene Names
SNAT; Serotonin acetylase; AA-NAT  [Similar Products]
UniProt Entry Name
SNAT_HUMAN
NCBI Summary for AANAT
The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Comments for AANAT
AA-NAT var1: Catalyzes the N-acetylation of serotonin into N- acetylserotonin. Controls the night/day rhythm of melatonin production in the pineal gland. Has a relative affinity for hydroxylated versus non-hydroxylated arylalkylamines. Belongs to the acetyltransferase family. AANAT subfamily. Monomer. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-31, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-205 site, the other YWHAZ monomer with similar effect.

Protein type: EC 2.3.1.87; Acetyltransferase; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: perinuclear region of cytoplasm; cytosol

Molecular Function: arylamine N-acetyltransferase activity; aralkylamine N-acetyltransferase activity

Biological Process: N-terminal protein amino acid acetylation; circadian rhythm; response to light stimulus; melatonin biosynthetic process; response to copper ion; indolalkylamine biosynthetic process; response to zinc ion; response to cytokine stimulus; response to calcium ion; response to corticosterone stimulus; response to insulin stimulus

Disease: Delayed Sleep Phase Syndrome, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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