• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

AAP elisa kit :: Chicken AAP ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2503632
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells  /  $235 +1 FREE 8GB USB
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
AAP elisa kit
Product Name

AAP, ELISA Kit

Full Product Name

Chicken AAP (Alanine Aminopeptidase) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
262850
3D Structure
ModBase 3D Structure for P08697
Species Reactivity
Specificity
This kit recognizes natural and recombinantChickenAAP. No significant cross-reactivity or interference between ChickenAAP and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL ; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AAPpurchase
MBS2503632 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AAP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AAP. The ELISA analytical biochemical technique of the MBS2503632 kit is based on AAP antibody-AAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AAP elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of ChickenAAPconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AAPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AAP.You can calculate the concentration of AAPin the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2503632. Click to request current manual
NCBI/Uniprot data below describe general gene information for AAP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,907 Da
NCBI Official Full Name
alpha-2-antiplasmin isoform a
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
NCBI Official Symbol
SERPINF2  [Similar Products]
NCBI Official Synonym Symbols
AAP; API; PLI; A2AP; ALPHA-2-PI
  [Similar Products]
NCBI Protein Information
alpha-2-antiplasmin; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin F2
UniProt Protein Name
Alpha-2-antiplasmin
UniProt Synonym Protein Names
Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
UniProt Gene Name
SERPINF2  [Similar Products]
UniProt Synonym Gene Names
AAP; PLI; Alpha-2-AP; Alpha-2-PI  [Similar Products]
UniProt Entry Name
A2AP_HUMAN
NCBI Summary for AAP
This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Comments for AAP
SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity

Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of cell differentiation

Disease: Alpha-2-plasmin Inhibitor Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with AAP elisa kit
 Reference Product  PubMed Publications
 PLAT elisa kit  >1 publications with AAP and PLAT
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000