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AAV elisa kit

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Catalog # MBS539692
Unit / Price
  96 Tests  /  $1,030 +1 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

AAV, ELISA Kit

Also Known As

AAV ELISA Kit

Product Synonym Names
AAV ELISA Kit; Adeno Associated Virus ELISA Kit; AAV Titration ELISA Kit
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Assay Type
Quantitative Sandwich
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AAV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAAVpurchase
MBS539692 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AAV, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AAV. The ELISA analytical biochemical technique of the MBS539692 kit is based on AAV antibody-AAV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AAV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AAV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AAV elisa kit
Principle of the Assay: The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV 2 capsids is coated onto microtiter strips and is used to capture AAV 2 particles from the specimen. Captured AAV particles are detected in two steps. First a biotin-conjugated monoclonal antibody to AAV 2 is bound to the immune complex. In the second step streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a color reaction which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm. The kit control provided contains an AAV 2 particle preparation of empty capsids. It shows a typical titration curve when used in dilutions of steps of two (Fig. 1). It allows the quantitative determination of samples of an unknown particle titre (immunological titer) and the calibration of an inhouse AAV 2 preparation (e.g. infectious titer, transducing units, DNA dot blot titer) (Table 1).

Background/Introduction: Adeno-associated virus (AAV) is a non pathogenic ssDNA virus that is a topic of intense study in gene therapy. The virus transduces a wide variety of dividing and non-dividing cells showing long-term gene expression with no cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinsons's, Cana????an disease) showing no serious vector-related adverse effects. Methods for the characterisation of AAV preparations currently include titration ELISA, realtime PCR, DNA dot blot, determination of transducing units, infectious center assay, SDSPAGE or electron microscopy. Immunotitration by AAV 2 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV 2 wt virions, AAV 2 recombinant virions or assembled and intact empty AAV 2 capsids.
Product Categories/Family for AAV elisa kit
Applications Tested/Suitable for AAV elisa kit
ELISA (EIA)

Typical Standard Curve/Testing Data of AAV elisa kit
AAV elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS539692. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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