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ABAT elisa kit :: Mouse 4-aminobutyrate aminotransferase, mitochondrial (ABAT) ELISA Kit

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Catalog # MBS1601089
Unit / Price
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  48-Strip-Wells  /  $280 +1 FREE 8GB USB
  96-Strip-Wells  /  $445 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,830 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,580 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

4-aminobutyrate aminotransferase, mitochondrial (ABAT), ELISA Kit

Full Product Name

Mouse 4-aminobutyrate aminotransferase, mitochondrial (ABAT) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.2ng/ml - 60ng/ml
Sensitivity
0.096ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ABAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ABATpurchase
MBS1601089 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 4-aminobutyrate aminotransferase, mitochondrial (ABAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABAT. The ELISA analytical biochemical technique of the MBS1601089 kit is based on ABAT antibody-ABAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ABAT elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse 4-aminobutyrate Aminotransferase, Mitochondrial (also known as ABAT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ABAT antibody. ABAT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ABAT Antibody is added and binds to ABAT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABAT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ABAT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Typical Testing Data/Standard Curve (for reference only) of ABAT elisa kit
ABAT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS1601089. Click to request current manual
NCBI/Uniprot data below describe general gene information for ABAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,439 Da
NCBI Official Full Name
4-aminobutyrate aminotransferase, partial
NCBI Official Synonym Full Names
4-aminobutyrate aminotransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
GABAT; NPD009; GABA-AT
  [Similar Products]
NCBI Protein Information
4-aminobutyrate aminotransferase, mitochondrial
UniProt Protein Name
4-aminobutyrate aminotransferase, mitochondrial
UniProt Synonym Protein Names
(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22); GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT
UniProt Gene Name
UniProt Synonym Gene Names
GABAT; GABA-AT; GABA transaminase; GABA-T  [Similar Products]
NCBI Summary for ABAT
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ABAT
Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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