• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ABCA1 elisa kit :: Porcine ABCA1 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2509972
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
ABCA1 elisa kit
Product Name

ABCA1, ELISA Kit

Full Product Name

Porcine ABCA1(ATP-binding cassette transporter A1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
205400
3D Structure
ModBase 3D Structure for O95477
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Porcine ABCA1. No significant cross-reactivity or interference between Porcine ABCA1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Porcine ABCA1 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ABCA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forABCA1purchase
MBS2509972 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ABCA1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABCA1. The ELISA analytical biochemical technique of the MBS2509972 kit is based on ABCA1 antibody-ABCA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABCA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABCA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ABCA1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ABCA1 in Porcine serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine ABCA1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine ABCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine ABCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine ABCA1. You can calculate the concentration of Porcine ABCA1 in the samples by comparing the OD of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for ABCA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
254,302 Da
NCBI Official Full Name
ATP-binding cassette sub-family A member 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family A (ABC1), member 1
NCBI Official Symbol
ABCA1  [Similar Products]
NCBI Official Synonym Symbols
TGD; ABC1; CERP; ABC-1; HDLDT1
  [Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein; membrane-bound
UniProt Protein Name
ATP-binding cassette sub-family A member 1
UniProt Synonym Protein Names
ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein
Protein Family
UniProt Gene Name
ABCA1  [Similar Products]
UniProt Synonym Gene Names
ABC1; CERP; ABC-1; ATP-binding cassette 1  [Similar Products]
UniProt Entry Name
ABCA1_HUMAN
NCBI Summary for ABCA1
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for ABCA1
ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Vesicle; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: Golgi apparatus; integral to plasma membrane; endocytic vesicle; perinuclear region of cytoplasm; plasma membrane; phagocytic vesicle; external side of plasma membrane; lipid raft

Molecular Function: apolipoprotein A-I receptor activity; ATPase activity; cholesterol binding; apolipoprotein binding; ATPase binding; apolipoprotein A-I binding; small GTPase binding; protein binding; syntaxin binding; phospholipid transporter activity; cholesterol transporter activity; phospholipid binding; anion transmembrane transporter activity; ATP binding; receptor binding

Biological Process: response to drug; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; intracellular cholesterol transport; cholesterol efflux; cellular lipid metabolic process; endosome transport; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; platelet dense granule organization and biogenesis; lysosome organization and biogenesis; response to low density lipoprotein stimulus; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; phagocytosis, engulfment; response to nutrient; phospholipid homeostasis; peptide secretion

Disease: Tangier Disease; Hypoalphalipoproteinemia, Primary; Hypercholesterolemia, Familial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000