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anti-Abl antibody :: Abl Antibody

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Catalog # MBS8504191
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

Abl, Antibody

Full Product Name

Abl (Phospho-Tyr393/412) Antibody

Product Synonym Names
Abelson murine leukemia viral oncogene 1; ABL1; c-ABL; EC 2.7.10.2; JTK7; p150; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
189980
3D Structure
ModBase 3D Structure for P00519
Species Reactivity
Human, Mouse, Rat
Specificity
Abl (Phospho-Tyr393/412) antibody detects endogenous levels of Abl only when phosphorylated at tyrosine 393/412.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Abl around the phosphorylation site of tyrosine 393/412 (D-T-YP-T-A).
All Sites
Human: Tyr393/Tyr439
Mouse: Tyr393/Tyr439
Rat: Tyr412/-
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-Abl antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Abl antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-Abl antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000

Testing Data of anti-Abl antibody
P-peptide-+
anti-Abl antibody Testing Data image
Immunohistochemistry (IHC) of anti-Abl antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Abl (Phospho-Tyr393/412) antibody. Western blot analysis of extracts from RAW264.7 cells, using Abl (Phospho-Tyr393/412) antibody.
anti-Abl antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-Abl antibody
P-peptide-+ Immunofluorescence analysis of A549 cells, using Abl (Phospho-Tyr393/412) antibody.
anti-Abl antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for Abl. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
124,955 Da
NCBI Official Full Name
tyrosine-protein kinase ABL1 isoform a
NCBI Official Synonym Full Names
ABL proto-oncogene 1, non-receptor tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
  [Similar Products]
NCBI Protein Information
tyrosine-protein kinase ABL1
UniProt Protein Name
Tyrosine-protein kinase ABL1
UniProt Synonym Protein Names
Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ABL; JTK7  [Similar Products]
NCBI Summary for Abl
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]
UniProt Comments for Abl
Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity ().
Product References and Citations for anti-Abl antibody
Li G.Y., Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. Nagase T., DNA Res. 6:63-70(1999). Krapivinsky G., Neuron 43:563-574(2004).

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