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ACE II elisa kit :: Human Angiotensin II Converting Enzyme ELISA Kit

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Catalog # MBS723213
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Angiotensin II Converting Enzyme (ACE II), ELISA Kit

Popular Item
Full Product Name

Human Angiotensin II Converting Enzyme ELISA Kit

Product Synonym Names
Human Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme; Angiotensin II Converting Enzyme (Human)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: X; NC_000023.10 (15579156..15620192, complement). Location: Xp22
OMIM
300335
3D Structure
ModBase 3D Structure for Q9BYF1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ACE2. No significant cross-reactivity or interference between ACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
50-1000pg/mL
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ACE II elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ACE IIpurchase
MBS723213 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin II Converting Enzyme (ACE II) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACE II. The ELISA analytical biochemical technique of the MBS723213 kit is based on ACE II antibody-ACE II antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACE II antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACE II. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ACE II elisa kit
Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACE2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACE2. Standards or samples are then added to the microtiter plate wells and ACE2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACE2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACE2 are added to each well to "sandwich" the ACE2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACE2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ACE II elisa kit

Typical Testing Data/Standard Curve (for reference only) of ACE II elisa kit
ACE II elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS723213. Click to request current manual
NCBI/Uniprot data below describe general gene information for ACE II. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92,463 Da
NCBI Official Full Name
angiotensin-converting enzyme 2
NCBI Official Synonym Full Names
angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
NCBI Official Symbol
NCBI Official Synonym Symbols
ACEH; DKFZp434A014
  [Similar Products]
NCBI Protein Information
angiotensin-converting enzyme 2; OTTHUMP00000022963; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog
UniProt Protein Name
Angiotensin-converting enzyme 2
UniProt Synonym Protein Names
ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15
UniProt Gene Name
UniProt Entry Name
ACE2_HUMAN
NCBI Summary for ACE II
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]
UniProt Comments for ACE II
Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Ref.1 Ref.2 Ref.14

Catalytic activity: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine.

Cofactor: Binds 1 zinc ion per subunit. Ref.13Binds 1 chloride ion per subunit. Ref.13

Enzyme regulation: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Ref.1 Ref.2 Ref.3 Ref.13

Subunit structure: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein. Ref.11 Ref.14 Ref.17 Ref.20 Ref.22

Subcellular location: Processed angiotensin-converting enzyme 2: Secreted Ref.3. Cell membrane; Single-pass type I membrane protein Ref.3.

Tissue specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. Ref.1 Ref.2 Ref.3 Ref.12 Ref.16 Ref.19

Induction: Up-regulated in failing heart. Ref.1 Ref.2 Ref.3 Ref.13 Ref.15 Ref.19

Post-translational modification: N-glycosylation on Asn-90 may limit SARS infectivity.

Sequence similarities: Belongs to the peptidase M2 family.

Biophysicochemical propertiesKinetic parameters:KM=6.9 µM for angiotensin I Ref.13KM=2 µM for angiotensin IIKM=6.8 µM for apelin-13KM=5.5 µM for dynorphin-13pH dependence:Optimum pH is 6.5 in the presence of 1 M NaCl. Active from pH 6 to 9.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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