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ACER2 elisa kit :: Mouse Alkaline ceramidase 2 (ACER2) ELISA Kit

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Catalog # MBS7209609
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Alkaline ceramidase 2 (ACER2), ELISA Kit

Popular Item
Full Product Name

Mouse Alkaline ceramidase 2 (ACER2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
613492
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ACER2. No significant cross-reactivity or interference between ACER2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACER2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ACER2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forACER2purchase
MBS7209609 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline ceramidase 2 (ACER2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACER2. The ELISA analytical biochemical technique of the MBS7209609 kit is based on ACER2 antibody-ACER2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACER2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACER2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ACER2 elisa kit
Intended Uses: This ACER2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACER2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACER2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER2 antibody and an ACER2-HRP conjugate. The assay sample and buffer are incubated together with ACER2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER2 concentration since ACER2 from samples and ACER2-HRP conjugate compete for the anti-ACER2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER2 from the sample, fewer sites are left to bind ACER2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER2 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of ACER2 elisa kit
ACER2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for ACER2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,864 Da
NCBI Official Full Name
alkaline ceramidase 2
NCBI Official Synonym Full Names
alkaline ceramidase 2
NCBI Official Symbol
ACER2  [Similar Products]
NCBI Official Synonym Symbols
ASAH3L; ALKCDase2
  [Similar Products]
NCBI Protein Information
alkaline ceramidase 2; haCER2; alkCDase 2; alkaline CDase 2; ceramide hydrolase
UniProt Protein Name
Alkaline ceramidase 2
UniProt Synonym Protein Names
Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like
Protein Family
UniProt Gene Name
ACER2  [Similar Products]
UniProt Synonym Gene Names
ASAH3L; PP11646; AlkCDase 2; Alkaline CDase 2; haCER2  [Similar Products]
UniProt Entry Name
ACER2_HUMAN
NCBI Summary for ACER2
The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010]
UniProt Comments for ACER2
ACER2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. Belongs to the alkaline ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - sphingolipid; EC 3.5.1.23; Hydrolase

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: Golgi membrane; Golgi apparatus; integral to Golgi membrane

Molecular Function: ceramidase activity

Biological Process: caspase activation; response to retinoic acid; sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; negative regulation of cell-matrix adhesion; ceramide metabolic process; negative regulation of cell adhesion mediated by integrin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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