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ACHE elisa kit :: Bovine acetylcholinesterase (Yt blood group) ELISA Kit

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Catalog # MBS908990
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

acetylcholinesterase (Yt blood group) (ACHE), ELISA Kit

Also Known As

Bovine Acetylcholinesterase, ACHE ELISA Kit

Product Synonym Names
Bovine Acetylcholinesterase (ACHE) ELISA kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS908990 Testing Data
Sequence Length
613
3D Structure
ModBase 3D Structure for P23795
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of bovine ACHE. No significant cross-reactivity or interference between bovine ACHE and analogues was observed.
Samples
Serum, plasma
Assay Type
Sandwich
Detection Range
0.9 4 U/ml -60 U/ml.
Sensitivity
The minimum detectable dose of bovine ACHE is typically less than 0.24 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ACHE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forACHEpurchase
MBS908990 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the acetylcholinesterase (Yt blood group) (ACHE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACHE. The ELISA analytical biochemical technique of the MBS908990 kit is based on ACHE antibody-ACHE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACHE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACHE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ACHE elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ACHE elisa kit
ACHE elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS908990. Click to request current manual
NCBI/Uniprot data below describe general gene information for ACHE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,664 Da
NCBI Official Full Name
acetylcholinesterase
NCBI Official Synonym Full Names
acetylcholinesterase
NCBI Official Symbol
NCBI Protein Information
acetylcholinesterase; acetylcholinesterase (Yt blood group)
UniProt Protein Name
Acetylcholinesterase
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ACES_BOVIN
UniProt Comments for ACHE
Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Catalytic activity: Acetylcholine + H2O = choline + acetate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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