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AChE elisa kit :: Rabbit Acetylcholinesterase ELISA Kit

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Catalog # MBS720206
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Acetylcholinesterase (AChE), ELISA Kit

Also Known As

Rabbit Acetylcholinesterase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene+112100
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AChE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAChEpurchase
MBS720206 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholinesterase (AChE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AChE. The ELISA analytical biochemical technique of the MBS720206 kit is based on AChE antibody-AChE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AChE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AChE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AChE elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This ACHE ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ACHE in the sample, this ACHE ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ACHE concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ACHE utilizes a multiclonal anti-ACHE antibody and an ACHE-HRP conjugate. The assay sample and buffer are incubated together with ACHE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACHE concentration since ACHE from samples and ACHE-HRP conjugate compete for the anti-ACHE antibody binding site. Since the number of sites is limited, as more sites are occupied by ACHE from the sample, fewer sites are left to bind ACHE-HRP conjugate. Standards of known ACHE concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ACHE. The ACHE concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AChE elisa kit

Typical Testing Data/Standard Curve (for reference only) of AChE elisa kit
AChE elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS720206. Click to request current manual
NCBI/Uniprot data below describe general gene information for AChE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
58,352 Da
NCBI Official Full Name
acetylcholinesterase
NCBI Official Synonym Full Names
acetylcholinesterase (Yt blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
YT; ACEE; ARACHE; N-ACHE
  [Similar Products]
NCBI Protein Information
acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
UniProt Protein Name
Acetylcholinesterase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ACES_HUMAN
NCBI Summary for AChE
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
UniProt Comments for AChE
ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Lipid Metabolism - glycerophospholipid; EC 3.1.1.7; Hydrolase

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; synapse; basal lamina; nucleus; cell junction; neuromuscular junction

Molecular Function: collagen binding; serine hydrolase activity; protein binding; protein homodimerization activity; protein self-association; cholinesterase activity; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: nervous system development; negative regulation of synaptic transmission, cholinergic; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; cell proliferation; synaptic transmission; synaptogenesis; positive regulation of protein secretion; retina development in camera-type eye; amyloid precursor protein metabolic process; receptor internalization; neurotransmitter receptor biosynthetic process; phospholipid metabolic process; phosphatidylcholine biosynthetic process; response to wounding; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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