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ACO1 elisa kit :: Human ACO1/Cytoplasmic Aconitate Hydratase ELISA Kit

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Catalog # MBS2890202
Unit / Price
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  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
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ACO1 elisa kit
Product Name

ACO1/Cytoplasmic Aconitate Hydratase, ELISA Kit

Full Product Name

Human ACO1/Cytoplasmic Aconitate Hydratase ELISA Kit

Product Synonym Names
Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; IREB1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
100880
3D Structure
ModBase 3D Structure for P21399
Species Reactivity
Specificity
Natural and recombinant Human Cytoplasmic aconitate hydratase
Assay Type
Sandwich
Detection Range
1.56-100U/L
Sensitivity
0.73U/L
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ACO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ACO1purchase
MBS2890202 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ACO1/Cytoplasmic Aconitate Hydratase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACO1. The ELISA analytical biochemical technique of the MBS2890202 kit is based on ACO1 antibody-ACO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for ACO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
cytoplasmic aconitate hydratase
NCBI Official Synonym Full Names
aconitase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
  [Similar Products]
NCBI Protein Information
cytoplasmic aconitate hydratase
UniProt Protein Name
Cytoplasmic aconitate hydratase
UniProt Synonym Protein Names
Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IREB1; Aconitase; IRP1; IRE-BP 1  [Similar Products]
UniProt Entry Name
ACOC_HUMAN
NCBI Summary for ACO1
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
UniProt Comments for ACO1
IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: Translation; RNA-binding; Lyase; Endoplasmic reticulum; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; RNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; aconitate hydratase activity

Biological Process: regulation of translation; cellular iron ion homeostasis; tricarboxylic acid cycle; citrate metabolic process; response to iron(II) ion; intestinal absorption; post-embryonic development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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