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ACOT8 elisa kit :: Monkey Acyl-coenzyme A thioesterase 8 (ACOT8) ELISA Kit

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Catalog # MBS7220560
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ACOT8 elisa kit
Product Name

Acyl-coenzyme A thioesterase 8 (ACOT8), ELISA Kit

Full Product Name

Monkey Acyl-coenzyme A thioesterase 8 (ACOT8) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
608123
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ACOT8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ACOT8purchase
MBS7220560 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acyl-coenzyme A thioesterase 8 (ACOT8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACOT8. The ELISA analytical biochemical technique of the MBS7220560 kit is based on ACOT8 antibody-ACOT8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACOT8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACOT8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for ACOT8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,914 Da[Similar Products]
NCBI Official Full Name
acyl-coenzyme A thioesterase 8
NCBI Official Synonym Full Names
acyl-CoA thioesterase 8
NCBI Official Symbol
ACOT8  [Similar Products]
NCBI Official Synonym Symbols
hTE; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III
  [Similar Products]
NCBI Protein Information
acyl-coenzyme A thioesterase 8; thioesterase II; thioesterase III; choloyl-CoA hydrolase; palmitoyl-CoA hydrolase; choloyl-coenzyme A thioesterase; long-chain fatty-acyl-CoA hydrolase; peroxisomal acyl-CoA thioesterase 1; HIV-Nef associated acyl-CoA thioesterase; peroxisomal long-chain acyl-CoA thioesterase 1; peroxisomal acyl-coenzyme A thioester hydrolase 1
UniProt Protein Name
Acyl-coenzyme A thioesterase 8
UniProt Synonym Protein Names
Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE
UniProt Gene Name
ACOT8  [Similar Products]
UniProt Synonym Gene Names
ACTEIII; PTE1; PTE2; Acyl-CoA thioesterase 8; PTE-1; hACTE-III; hACTEIII; hTE  [Similar Products]
UniProt Entry Name
ACOT8_HUMAN
NCBI Summary for ACOT8
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
UniProt Comments for ACOT8
ACOT8: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs. May be involved in the metabolic regulation of peroxisome proliferation. Belongs to the C/M/P thioester hydrolase family.

Protein type: EC 3.1.2.27; Hydrolase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: peroxisomal matrix; mitochondrion

Molecular Function: protein binding; acyl-CoA hydrolase activity; choloyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: peroxisome fission; bile acid biosynthetic process; fatty acid beta-oxidation using acyl-CoA oxidase; acyl-CoA metabolic process; viral reproduction; bile acid metabolic process; unsaturated fatty acid metabolic process; cellular lipid metabolic process; dicarboxylic acid catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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