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ACTa1 clia kit :: Human ACTalpha1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit

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Catalog # MBS2532453
Unit / Price
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  48-Strip-Wells  /  $450 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

ACTalpha1 (Actin Alpha 1, Skeletal Muscle) (ACTa1), CLIA Kit

Full Product Name

Human ACTalpha1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit

Product Synonym Names
ACTA1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Human ACTa1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit; Human ACTa1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
102610
Species Reactivity
Specificity
This kit recognizes natural and recombinant ACTalpha1. No significant cross-reactivity or interference between ACTalpha1 and analogues was observed.
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ACTa1 clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACTa1 clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of ACTalpha1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ACTalpha1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACTalpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACTalpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ACTalpha1. You can calculate the concentration of ACTalpha1 in the samples by comparing the RLU value of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ACTa1 clia kit
ACTa1 clia kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2532453. Click to request current manual
NCBI/Uniprot data below describe general gene information for ACTa1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,051 Da
NCBI Official Full Name
actin alpha 1 skeletal muscle protein
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1  [Similar Products]
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
  [Similar Products]
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Synonym Protein Names
Alpha-actin-1
UniProt Gene Name
ACTA1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ACTS_HUMAN
NCBI Summary for ACTa1
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
UniProt Comments for ACTa1
ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Contractile; Cell development/differentiation; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; lamellipodium; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton; filopodium

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to mechanical stimulus; response to steroid hormone stimulus; muscle thin filament assembly; response to extracellular stimulus; response to lithium ion; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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