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anti-ACTB antibody :: Mouse beta-Actin Antibody

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Catalog # MBS850843
Unit / Price
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  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

beta-Actin (ACTB), Antibody

Full Product Name

beta-Actin Antibody

Product Synonym Names
PS1TP5BP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
102630
3D Structure
ModBase 3D Structure for P60709
Isotype
IgG2b
Clone Number
8H10D10
Host
Mouse
Species Reactivity
Human, Mouse, Hamster, Rat, Monkey
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Immunogen
Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Other Notes
Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTB antibody
Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Applications Tested/Suitable for anti-ACTB antibody
Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-ACTB antibody
WB: 1:500-1:2000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000

Western Blot (WB) of anti-ACTB antibody
Figure 1. Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
anti-ACTB antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-ACTB antibody
Figure 2. Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue. DRAQ5 fluorescent DNA dye.
anti-ACTB antibody Immunofluorescence (IF) image
Flow Cytometry (FC/FACS) of anti-ACTB antibody
Figure 3. Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
anti-ACTB antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42kDa
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
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NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
UniProt Gene Name
NCBI Summary for ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
UniProt Comments for ACTB
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.CautionWas originally thought to be part of the MLL5-L complex, at least composed of KMT2E, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT (PubMed:19377461). However, the corresponding article has been retracted (PubMed:24336203).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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