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ADAM10 elisa kit :: Porcine A Disintegrin And Metalloprotease 10 ELISA Kit

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Catalog # MBS741939
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

A Disintegrin And Metalloprotease 10 (ADAM10), ELISA Kit

Popular Item
Full Product Name

Porcine A Disintegrin And Metalloprotease 10 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
615590
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAM10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADAM10purchase
MBS741939 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM10. The ELISA analytical biochemical technique of the MBS741939 kit is based on ADAM10 antibody-ADAM10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAM10 elisa kit
Intended Uses: This ADAM10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ADAM10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from samples and ADAM10-HRP conjugate compete for the anti-ADAM10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM10 from the sample, fewer sites are left to bind ADAM10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM10 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADAM10 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ADAM10 elisa kit
ADAM10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS741939. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
84,142 Da
NCBI Official Full Name
ADAM10
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
ADAM10  [Similar Products]
NCBI Official Synonym Symbols
RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Synonym Protein Names
CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD_antigen: CD156c
UniProt Gene Name
ADAM10  [Similar Products]
UniProt Synonym Gene Names
KUZ; MADM; ADAM 10  [Similar Products]
UniProt Entry Name
ADA10_HUMAN
NCBI Summary for ADAM10
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]
UniProt Comments for ADAM10
ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: EC 3.4.24.81; Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Vesicle; Cell surface; Protease

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; focal adhesion; cell surface; intracellular membrane-bound organelle; membrane; postsynaptic density; cytoplasm; plasma membrane; integral to membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; axon guidance; Notch signaling pathway; extracellular matrix organization and biogenesis; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; protein amino acid phosphorylation; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; cell-cell signaling; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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