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ADAM10 recombinant protein :: A Disintegrin And Metalloprotease 10 (ADAM10) Recombinant Protein

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Catalog # MBS2009286 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $150 +1 FREE 8GB USB
  0.05 mg  /  $285 +1 FREE 8GB USB
  0.1 mg  /  $445 +1 FREE 8GB USB
  0.2 mg  /  $550 +1 FREE 8GB USB
  0.5 mg  /  $1,060 +1 FREE 8GB USB
  1 mg  /  $1,580 +1 FREE 8GB USB
SDS-Page
Product Name

A Disintegrin And Metalloprotease 10 (ADAM10), Recombinant Protein

Popular Item
Full Product Name

Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-TSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGKK CKLKPGKQCS PSQGPCCTAQ CAFKSKSEKC RDDSDCAKEG ICNGFTALCP ASDPKPNFTD CNRHTQVCIN GQCAGSICEK YDLEECTCAS SDGKDDKELC HVCCMKKMAP STCASTGSLQ WNKQFTGRTI TLQPGSPCND FRGYCDVFMR CRLVD
Chromosome Location
Chromosome: 8; NC_005107.3 (75842118..75974564, complement). Location: 8q24
3D Structure
ModBase 3D Structure for Q10743
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Thr218~Asp445 (Accession # Q10743) with N-terminal His-Tag
Subcellular Location
Membrane; Single-pass type I membrane protein
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.4
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of ADAM10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADAM10 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for ADAM10 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
26.5kDa
NCBI Official Full Name
Disintegrin and metalloproteinase domain-containing protein 10
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
Adam10  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metallopeptidase domain 10
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Synonym Protein Names
Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease
UniProt Gene Name
Adam10  [Similar Products]
UniProt Synonym Gene Names
Madm; ADAM 10  [Similar Products]
UniProt Entry Name
ADA10_RAT
NCBI Summary for ADAM10
may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006]
UniProt Comments for ADAM10
ADAM10: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling (By similarity)

Protein type: Motility/polarity/chemotaxis; Protease; Vesicle; EC 3.4.24.81; Cell surface; Membrane protein, integral; Cell adhesion

Cellular Component: Golgi apparatus; focal adhesion; cell surface; intracellular membrane-bound organelle; membrane; postsynaptic density; cytoplasm; integral to membrane; Golgi-associated vesicle; trans-Golgi network; nucleus

Molecular Function: protein homodimerization activity; zinc ion binding; metallopeptidase activity; metal ion binding; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding

Biological Process: PMA-inducible membrane protein ectodomain proteolysis; Notch signaling pathway; regulation of osteoclast differentiation; monocyte activation; in utero embryonic development; membrane protein ectodomain proteolysis; positive regulation of cell proliferation; constitutive protein ectodomain proteolysis; negative regulation of cell adhesion; proteolysis; positive regulation of cell growth; protein amino acid phosphorylation; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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