• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ADAM12 elisa kit :: Human ADAM metallopeptidase domain 12 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS704578
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $575 +1 FREE 8GB USB
  96-Strip-Wells  /  $835 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ADAM metallopeptidase domain 12 (ADAM12), ELISA Kit

Popular Item
Also Known As

Human A Disintegrin And Metalloprotease 12, ADAM12 ELISA Kit

Product Synonym Names
Human A Disintegrin And Metalloprotease 12; ADAM12 ELISA Kit; RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha) ; meltrin alpha; ADAM metallopeptidase domain 12
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704578 Testing Data
Sequence Length
737
OMIM
602714
3D Structure
ModBase 3D Structure for O43184
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ADAM12. No significant cross-reactivity or interference between Human ADAM12 and analogues was observed.
Samples
Serum, plasma, urine, cell culture supernates, tissue homogenates, Cell lysates
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Adhesion
Protein Biological Process 3
Cell adhesion
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAM12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADAM12purchase
MBS704578 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase domain 12 (ADAM12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM12. The ELISA analytical biochemical technique of the MBS704578 kit is based on ADAM12 antibody-ADAM12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAM12 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADAM12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADAM12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ADAM12 elisa kit
ADAM12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704578. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAM12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
99,542 Da
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 12 isoform 4 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 12
NCBI Official Symbol
ADAM12  [Similar Products]
NCBI Official Synonym Symbols
MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 12
UniProt Synonym Protein Names
Meltrin-alpha
UniProt Gene Name
ADAM12  [Similar Products]
UniProt Synonym Gene Names
MLTN; UNQ346/PRO545; ADAM 12  [Similar Products]
UniProt Entry Name
ADA12_HUMAN
NCBI Summary for ADAM12
This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
UniProt Comments for ADAM12
Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors

Cofactor: Binds 1 zinc ion per subunit.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000