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ADAM8 elisa kit :: Goat A Disintegrin And Metalloprotease 8 ELISA Kit

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Catalog # MBS741160
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

A Disintegrin And Metalloprotease 8 (ADAM8), ELISA Kit

Full Product Name

Goat A Disintegrin And Metalloprotease 8 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602267
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ADAM8. No significant cross-reactivity or interference between ADAM8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM8 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Intended Uses
This ADAM8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat ADAM8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAM8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADAM8purchase
MBS741160 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM8. The ELISA analytical biochemical technique of the MBS741160 kit is based on ADAM8 antibody-ADAM8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAM8 elisa kit
Principle of the assay: ADAM8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM8 antibody and an ADAM8-HRP conjugate. The assay sample and buffer are incubated together with ADAM8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM8 concentration since ADAM8 from samples and ADAM8-HRP conjugate compete for the anti-ADAM8 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM8 from the sample, fewer sites are left to bind ADAM8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM8 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADAM8 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ADAM8 elisa kit
ADAM8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS741160. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAM8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,262 Da
NCBI Official Full Name
ADAM8 protein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 8
NCBI Official Symbol
ADAM8  [Similar Products]
NCBI Official Synonym Symbols
MS2; CD156; CD156a
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 8
UniProt Synonym Protein Names
Cell surface antigen MS2; CD_antigen: CD156a
UniProt Gene Name
ADAM8  [Similar Products]
UniProt Synonym Gene Names
MS2; ADAM 8  [Similar Products]
UniProt Entry Name
ADAM8_HUMAN
NCBI Summary for ADAM8
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]
UniProt Comments for ADAM8
ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; cell adhesion molecule binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; extracellular matrix disassembly; positive regulation of protein kinase B signaling cascade; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; lymphocyte chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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