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ADAMTS14 elisa kit :: Human A disintegrin and metalloproteinase with thrombospondin motifs 14 (ADAMTS14) ELISA Kit

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Catalog # MBS7207540
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

A disintegrin and metalloproteinase with thrombospondin motifs 14 (ADAMTS14), ELISA Kit

Also Known As

Human A disintegrin and metalloproteinase with thrombospondin motifs 14 (ADAMTS14) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607506
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ADAMTS14. No significant cross-reactivity or interference between ADAMTS14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS14 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAMTS14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADAMTS14purchase
MBS7207540 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A disintegrin and metalloproteinase with thrombospondin motifs 14 (ADAMTS14) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS14. The ELISA analytical biochemical technique of the MBS7207540 kit is based on ADAMTS14 antibody-ADAMTS14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAMTS14 elisa kit
Intended Uses: This ADAMTS14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTS14. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ADAMTS14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADAMTS14 antibody and an ADAMTS14-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS14 concentration since ADAMTS14 from samples and ADAMTS14-HRP conjugate compete for the anti-ADAMTS14 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS14 from the sample, fewer sites are left to bind ADAMTS14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS14 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADAMTS14 elisa kit

Typical Standard Curve/Testing Data of ADAMTS14 elisa kit
ADAMTS14 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7207540. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAMTS14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
134,205 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 14 isoform 1 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 14
NCBI Official Symbol
ADAMTS14  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 14; ADAM-TS14; ADAMTS-14; ADAM-TS 14; metalloprotease-disintegrin protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 14
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 14
UniProt Gene Name
ADAMTS14  [Similar Products]
UniProt Synonym Gene Names
ADAM-TS 14; ADAM-TS14; ADAMTS-14  [Similar Products]
UniProt Entry Name
ATS14_HUMAN
NCBI Summary for ADAMTS14
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. This gene is highly similar to two family members, ADAMTS2 and ADAMTS3, in its sequence and gene structure, and the encoded protein shares the aminoprocollagen peptidase activity with the protein products encoded by ADAMTS2 and ADAMTS3. Various transcript variants of this gene have been identified. They result from the use of two different promoters and transcription initiation sites as well as alternative splicing sites. The full length nature of some transcripts has not been defined. [provided by RefSeq, Jul 2008]
UniProt Comments for ADAMTS14
ADAMTS14: Has a aminoprocollagen type I activity processing activity in the absence of ADAMTS2. Seems to be synthesized as a latent enzyme that requires activation to display aminoprocollagen peptidase activity. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.24.-; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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