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ADAMTS17 elisa kit :: Bovine A disintegrin and metalloproteinase with thrombospondin motifs 17 (ADAMTS17) ELISA Kit

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Catalog # MBS7201609
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

A disintegrin and metalloproteinase with thrombospondin motifs 17 (ADAMTS17), ELISA Kit

Full Product Name

Bovine A disintegrin and metalloproteinase with thrombospondin motifs 17 (ADAMTS17) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613195
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ADAMTS17. No significant cross-reactivity or interference between ADAMTS17 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS17 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This ADAMTS17 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ADAMTS17. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAMTS17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ADAMTS17purchase
MBS7201609 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A disintegrin and metalloproteinase with thrombospondin motifs 17 (ADAMTS17) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS17. The ELISA analytical biochemical technique of the MBS7201609 kit is based on ADAMTS17 antibody-ADAMTS17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAMTS17 elisa kit
Priciple of the assay: ADAMTS17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMTS17 antibody and an ADAMTS17-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS17 concentration since ADAMTS17 from samples and ADAMTS17-HRP conjugate compete for the anti-ADAMTS17 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS17 from the sample, fewer sites are left to bind ADAMTS17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS17 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADAMTS17 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ADAMTS17 elisa kit
ADAMTS17 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7201609. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAMTS17. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,757 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 17 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 17
NCBI Official Symbol
ADAMTS17  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 17; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 17
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 17
UniProt Gene Name
ADAMTS17  [Similar Products]
UniProt Synonym Gene Names
ADAM-TS 17; ADAM-TS17; ADAMTS-17  [Similar Products]
UniProt Entry Name
ATS17_HUMAN
NCBI Summary for ADAMTS17
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the protein encoded by ADAMTS19, another family member. The function of this protein has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for ADAMTS17
ADAMTS17: Defects in ADAMTS17 are the cause of Weill-Marchesani- like syndrome (WMLS). It is a disorder characterized by many of the key features of Weill-Marchesani syndrome, including lenticular myopia, ectopia lentis, glaucoma, spherophakia and short stature. However, the characteristic brachydactyly or decreased joint flexibility of Weill-Marchesani syndrome are absent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis

Disease: Weill-marchesani-like Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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