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ADAMTS5 elisa kit :: Human A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit

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Catalog # MBS2020569 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $665 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5), ELISA Kit

Popular Item
Also Known As

A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit

Product Synonym Names
ADMP-2; Aggrecanase-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
930
OMIM
605007
3D Structure
ModBase 3D Structure for Q9UNA0
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ADAMTS5. No significant cross-reactivity or interference between ADAMTS5 and analogues was observed.
Samples
human serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
<0.127ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADAMTS5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADAMTS5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAMTS5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADAMTS5purchase
MBS2020569 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS5. The ELISA analytical biochemical technique of the MBS2020569 kit is based on ADAMTS5 antibody-ADAMTS5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAMTS5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADAMTS5 in human serum, plasma, tissue homogenates and other biological fluids.
Product Categories/Family for ADAMTS5 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ADAMTS5 elisa kit
ADAMTS5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020569. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAMTS5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
101,718 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 5 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 5
NCBI Official Symbol
ADAMTS5  [Similar Products]
NCBI Official Synonym Symbols
ADMP-2; ADAM-TS5; ADAMTS-5; ADAMTS11; ADAM-TS 5; ADAMTS-11; ADAM-TS 11
  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 5
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
UniProt Synonym Protein Names
A disintegrin and metalloproteinase with thrombospondin motifs 11; ADAM-TS 11; ADAMTS-11; ADMP-2; Aggrecanase-2
UniProt Gene Name
ADAMTS5  [Similar Products]
UniProt Synonym Gene Names
ADAMTS11; ADMP2; ADAM-TS 5; ADAM-TS5; ADAMTS-5; ADAM-TS 11; ADAMTS-11  [Similar Products]
UniProt Entry Name
ATS5_HUMAN
NCBI Summary for ADAMTS5
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq, Jul 2008]
UniProt Comments for ADAMTS5
ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Protein type: EC 3.4.24.-; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region

Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: extracellular matrix disassembly; protein amino acid O-linked glycosylation; extracellular matrix organization and biogenesis; cellular protein metabolic process; defense response to bacterium; post-translational protein modification; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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