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ADAMTS5 elisa kit :: Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 ELISA Kit

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Catalog # MBS911846
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), ELISA Kit

Popular Item
Also Known As

Human A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit

Product Synonym Names
Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAM metallopeptidase with thrombospondin type 1 motif; 5
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS911846 Testing Data
Sequence Length
207
3D Structure
ModBase 3D Structure for Q9TT92
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ADAMTS5. No significant cross-reactivity or interference between Human ADAMTS5 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 ng/ml-1000 ng/ml
Sensitivity
3.9 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAMTS5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADAMTS5purchase
MBS911846 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS5. The ELISA analytical biochemical technique of the MBS911846 kit is based on ADAMTS5 antibody-ADAMTS5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAMTS5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ADAMTS5 elisa kit
ADAMTS5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS911846. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAMTS5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
22,576 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 5
NCBI Official Symbol
ADAMTS5  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAM-TS 5; aggrecanase-2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
UniProt Synonym Protein Names
ADMP-2; Aggrecanase-2
UniProt Gene Name
ADAMTS5  [Similar Products]
UniProt Synonym Gene Names
ADAM-TS 5; ADAM-TS5; ADAMTS-5  [Similar Products]
UniProt Entry Name
ATS5_BOVIN
UniProt Comments for ADAMTS5
Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Catalytic activity: Cleaves aggrecan at the 392-Glu-|-Ala-393 site.

Cofactor: Binds 1 zinc ion per subunit
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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