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ADAMTS8 elisa kit :: Human A Disintegrin And Metalloproteinase With Thrombospondin 8 ELISA Kit

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Catalog # MBS2533412
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Typical Testing Data/Standard Curve (for reference only)
Product Name

A Disintegrin And Metalloproteinase With Thrombospondin 8 (ADAMTS8), ELISA Kit

Popular Item
Full Product Name

Human ADAMTS8 (A Disintegrin And Metalloproteinase With Thrombospondin 8) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605175
Species Reactivity
Specificity
This kit recognizes natural and recombinantHumanADAMTS8. No significant cross-reactivity or interference between HumanADAMTS8 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAMTS8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ADAMTS8purchase
MBS2533412 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloproteinase With Thrombospondin 8 (ADAMTS8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS8. The ELISA analytical biochemical technique of the MBS2533412 kit is based on ADAMTS8 antibody-ADAMTS8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADAMTS8 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanADAMTS8concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADAMTS8. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADAMTS8and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADAMTS8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADAMTS8.You can calculate the concentration of ADAMTS8in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ADAMTS8 elisa kit
ADAMTS8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533412. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADAMTS8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96,460 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 8 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 8
NCBI Official Symbol
ADAMTS8  [Similar Products]
NCBI Official Synonym Symbols
METH2; ADAM-TS8
  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 8
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 8
UniProt Synonym Protein Names
METH-2; METH-8
UniProt Gene Name
ADAMTS8  [Similar Products]
UniProt Synonym Gene Names
METH2; ADAM-TS 8; ADAM-TS8; ADAMTS-8  [Similar Products]
UniProt Entry Name
ATS8_HUMAN
NCBI Summary for ADAMTS8
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene, most notably lung neoplasms. Reduced expression of this gene has been observed in multiple human cancers and this gene has been proposed as a potential tumor suppressor. [provided by RefSeq, Feb 2016]
UniProt Comments for ADAMTS8
ADAMTS8: Has anti-angiogenic properties.

Protein type: Secreted; EC 3.4.24.-; Motility/polarity/chemotaxis; Cell cycle regulation; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; low affinity phosphate transmembrane transporter activity; metalloendopeptidase activity; metallopeptidase activity; zinc ion binding

Biological Process: negative regulation of cell proliferation; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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