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ADCY10 elisa kit :: Canine Adenylate cyclase type 10 (ADCY10) ELISA Kit

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Catalog # MBS7203713
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ADCY10 elisa kit
Product Name

Adenylate cyclase type 10 (ADCY10), ELISA Kit

Full Product Name

Canine Adenylate cyclase type 10 (ADCY10) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605205
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADCY10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ADCY10purchase
MBS7203713 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenylate cyclase type 10 (ADCY10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADCY10. The ELISA analytical biochemical technique of the MBS7203713 kit is based on ADCY10 antibody-ADCY10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADCY10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADCY10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for ADCY10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
169,750 Da
NCBI Official Full Name
adenylate cyclase type 10 isoform 1
NCBI Official Synonym Full Names
adenylate cyclase 10 (soluble)
NCBI Official Symbol
ADCY10  [Similar Products]
NCBI Official Synonym Symbols
SAC; HCA2; SACI; Sacy; hsAC
  [Similar Products]
NCBI Protein Information
adenylate cyclase type 10; AH-related protein; ATP pyrophosphate-lyase; 3',5'-cyclic AMP synthetase; germ cell soluble adenylyl cyclase; testicular soluble adenylyl cyclase (SAC)
UniProt Protein Name
Adenylate cyclase type 10
UniProt Synonym Protein Names
AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase
Protein Family
UniProt Gene Name
ADCY10  [Similar Products]
UniProt Synonym Gene Names
SAC; hsAC; sAC  [Similar Products]
UniProt Entry Name
ADCYA_HUMAN
NCBI Summary for ADCY10
The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]
UniProt Comments for ADCY10
SAC: Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation. Genetic variations in ADCY10 are a cause of susceptibility to hypercalciuria absorptive type 2 (HCA2). Absorptive hypercalciuria is a common type of hypercalciuria, a condition characterized by excessive urinary calcium excretion. Absorptive hypercalciuria is due to gastrointestinal hyperabsorption of calcium and is a frequent cause of calcium oxalate nephrolithiasis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Nucleotide Metabolism - purine; EC 4.6.1.1; Adenylyl cyclase

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: growth cone; cytoskeleton; cell soma; apical part of cell; axon; perinuclear region of cytoplasm; dendrite; cytoplasm; plasma membrane; cytosol

Molecular Function: manganese ion binding; magnesium ion binding; ATP binding; ATPase binding; adenylate cyclase activity

Biological Process: positive regulation of apoptosis; spermatogenesis; cAMP biosynthetic process

Disease: Hypercalciuria, Absorptive, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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