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ADCY10 elisa kit :: Human Adenylate Cyclase 10, Soluble (ADCY10) ELISA Kit

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Catalog # MBS456935
Unit / Price
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  48-Strip-Wells  /  $385 +1 FREE 8GB USB
  96-Strip-Wells  /  $515 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,325 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,515 +5 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Adenylate Cyclase 10, Soluble (ADCY10), ELISA Kit

Full Product Name

Human Adenylate Cyclase 10, Soluble (ADCY10) ELISA Kit

Product Synonym Names
SAC; HCA2; SACI; Sacy; Testicular soluble adenylyl cyclase; Hypercalciuria,Absorptive 2; AH-related protein; Germ cell soluble adenylyl cyclase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
AF271058 mRNA
3D Structure
ModBase 3D Structure for Q96PN6
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ADCY10. No significant cross-reactivity or interference between ADCY10 and analogues was observed.
Samples
Tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.051ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADCY10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADCY10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADCY10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ADCY10purchase
MBS456935 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenylate Cyclase 10, Soluble (ADCY10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADCY10. The ELISA analytical biochemical technique of the MBS456935 kit is based on ADCY10 antibody-ADCY10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADCY10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADCY10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADCY10 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ADCY10 in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADCY10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADCY10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADCY10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADCY10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ADCY10 elisa kit
ADCY10 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS456935. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADCY10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
169,750 Da
NCBI Official Full Name
adenylate cyclase type 10 isoform 2
NCBI Official Synonym Full Names
adenylate cyclase 10
NCBI Official Symbol
ADCY10  [Similar Products]
NCBI Official Synonym Symbols
SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
  [Similar Products]
NCBI Protein Information
adenylate cyclase type 10
UniProt Protein Name
Adenylate cyclase type 10
UniProt Synonym Protein Names
AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase
Protein Family
UniProt Gene Name
ADCY10  [Similar Products]
UniProt Synonym Gene Names
SAC; hsAC; sAC  [Similar Products]
NCBI Summary for ADCY10
The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]
UniProt Comments for ADCY10
Catalyzes the formation of the signaling molecule cAMP (PubMed:12609998, PubMed:15659711, PubMed:24616449, PubMed:25040695, PubMed:24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO2 levels (PubMed:15659711, PubMed:17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (). Involved in ciliary beat regulation (PubMed:17591988).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with ADCY10 elisa kit
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