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ADCYAP1 elisa kit :: Rat adenylate cyclase activating polypeptide 1 (pituitary) ELISA Kit

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Catalog # MBS706315
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
ADCYAP1 elisa kit
Product Name

adenylate cyclase activating polypeptide 1 (pituitary) (ADCYAP1), ELISA Kit

Also Known As

Rat Pituitary adenylate cyclase-activating polypeptide, ADCYAP1 ELISA Kit

Product Synonym Names
Rat Pituitary adenylate cyclase-activating polypeptide (ADCYAP1) ELISA kit; MGC126852; PACAP; OTTHUMP00000162201; adenylate cyclase activating polypeptide; adenylate cyclase activating polypeptide 1 (pituitary)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
175
3D Structure
ModBase 3D Structure for P13589
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat ADCYAP1. No significant cross-reactivity or interference between Rat ADCYAP1 and analogues was observed.
Assay Type
Competitive
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADCYAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADCYAP1purchase
MBS706315 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenylate cyclase activating polypeptide 1 (pituitary) (ADCYAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADCYAP1. The ELISA analytical biochemical technique of the MBS706315 kit is based on ADCYAP1 antibody-ADCYAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADCYAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADCYAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADCYAP1 elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ADCYAP1 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated ADCYAP1 and incubated. A competitive inhibition reaction is launched between ADCYAP1 (Standards or samples) and Biotin-conjugated ADCYAP1 with the pre-coated antibody specific for ADCYAP1. The more amount of ADCYAP1 in samples, the less antibody bound by Biotin-conjugated ADCYAP1. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of ADCYAP1 in the sample. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS706315. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADCYAP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
19,557 Da
NCBI Official Full Name
pituitary adenylate cyclase-activating polypeptide
NCBI Official Synonym Full Names
adenylate cyclase activating polypeptide 1
NCBI Official Symbol
Adcyap1  [Similar Products]
NCBI Official Synonym Symbols
Pacap
  [Similar Products]
NCBI Protein Information
pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase activating polypeptide 1; adenylate cyclase activating polypeptide 1 (pituitary); pituitary adenylate cyclase-activating polypeptide precursor (Pacap)
UniProt Protein Name
Pituitary adenylate cyclase-activating polypeptide
UniProt Synonym Protein Names
PRP-48
UniProt Gene Name
Adcyap1  [Similar Products]
UniProt Synonym Gene Names
PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38  [Similar Products]
UniProt Entry Name
PACA_RAT
NCBI Summary for ADCYAP1
regulatory protein that stimulates cell proliferation, induces differentiation, modulates neurotransmitter release, vasodilation, bronchodilation, and hormone release, and suppresses inflammatory responses [RGD, Feb 2006]
UniProt Comments for ADCYAP1
Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.1 PublicationManual assertion based on experiment in:Ref.4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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