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ADH/VP/AVP elisa kit :: Mouse diuretic hormone/vasopressin/arginine vasopressin ELISA Kit

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Catalog # MBS725028
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

diuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP), ELISA Kit

Popular Item
Also Known As

Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

Product Synonym Names
Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit; antidiuretic hormone/vasopressin/arginine vasopressin; antidiuretic hormone/vasopressin/arginine vasopressin (Mouse)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 20; NC_000020.10 (3063202..3065370, complement). Location: 20p13
OMIM
125700
3D Structure
ModBase 3D Structure for P01185
Species Reactivity
Specificity
Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Specificity: This assay has high sensitivity and excellent specificity for detection of ADH. No significant cross-reactivity or interference between ADH and analogues was observed.
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADH/VP/AVP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADH/VP/AVPpurchase
MBS725028 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the diuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADH/VP/AVP. The ELISA analytical biochemical technique of the MBS725028 kit is based on ADH/VP/AVP antibody-ADH/VP/AVP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH/VP/AVP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH/VP/AVP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADH/VP/AVP elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma

Intended Uses: This ADH ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ADH in the sample, this ADH ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ADH concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ADH utilizes a polyclonal anti-ADH antibody and an ADH-HRP conjugate. The assay sample and buffer are incubated together with ADH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH concentration since ADH from samples and ADH-HRP conjugate compete for the anti-ADH antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH from the sample, fewer sites are left to bind ADH-HRP conjugate. Standards of known ADH concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of ADH. The ADH concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADH/VP/AVP elisa kit

Typical Testing Data/Standard Curve (for reference only) of ADH/VP/AVP elisa kit
ADH/VP/AVP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS725028. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADH/VP/AVP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,325 Da
NCBI Official Full Name
Arginine vasopressin
NCBI Official Synonym Full Names
arginine vasopressin
NCBI Official Symbol
NCBI Official Synonym Symbols
VP; ADH; ARVP; AVRP; AVP-NPII
  [Similar Products]
NCBI Protein Information
vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II
UniProt Protein Name
Vasopressin-neurophysin 2-copeptin
UniProt Synonym Protein Names
AVP-NPIICleaved into the following 3 chains:Arg-vasopressin; Alternative name(s):; Arginine-vasopressinNeurophysin 2; Alternative name(s):; Neurophysin-II
UniProt Gene Name
UniProt Synonym Gene Names
ARVP; VP  [Similar Products]
UniProt Entry Name
NEU2_HUMAN
NCBI Summary for ADH/VP/AVP
This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]
UniProt Comments for ADH/VP/AVP
Function: Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.

Subcellular location: Secreted.

Involvement in disease: Diabetes insipidus, neurohypophyseal (NDI) [MIM:125700]: A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopressin deficiency during childhood.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36

Sequence similarities: Belongs to the vasopressin/oxytocin family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with ADH/VP/AVP elisa kit
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