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ADI1 elisa kit :: Human 1, 2 dihydroxy 3 keto 5 methylthiopentene dioxygenase (ADI1) ELISA Kit

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Catalog # MBS7213177
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ADI1 elisa kit
Product Name

1, 2 dihydroxy 3 keto 5 methylthiopentene dioxygenase (ADI1), ELISA Kit

Popular Item
Full Product Name

Human 1, 2 dihydroxy 3 keto 5 methylthiopentene dioxygenase (ADI1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
613400
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADI1purchase
MBS7213177 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1, 2 dihydroxy 3 keto 5 methylthiopentene dioxygenase (ADI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADI1. The ELISA analytical biochemical technique of the MBS7213177 kit is based on ADI1 antibody-ADI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for ADI1 elisa kit
NCBI/Uniprot data below describe general gene information for ADI1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,332 Da[Similar Products]
NCBI Official Full Name
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
NCBI Official Synonym Full Names
acireductone dioxygenase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ARD; APL1; SIPL; mtnD; Fe-ARD; MTCBP1; Ni-ARD; HMFT1638
  [Similar Products]
NCBI Protein Information
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; submergence induced protein 2; submergence-induced protein-like factor; MT1-MMP cytoplasmic tail-binding protein-1; acireductone dioxygenase (Fe(2+)-requiring); acireductone dioxygenase (Ni(2+)-requiring); membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1
UniProt Protein Name
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
UniProt Synonym Protein Names
Acireductone dioxygenase (Fe(2+)-requiring); ARD; Fe-ARD; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; MTCBP-1; Submergence-induced protein-like factor; Sip-L
UniProt Gene Name
UniProt Synonym Gene Names
MTCBP1; HMFT1638; ARD; Fe-ARD; MTCBP-1; Sip-L  [Similar Products]
UniProt Entry Name
MTND_HUMAN
NCBI Summary for ADI1
This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. [provided by RefSeq, May 2010]
UniProt Comments for ADI1
ADI1: Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. Belongs to the acireductone dioxygenase (ARD) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.13.11.54; Oxidoreductase; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: acireductone dioxygenase [iron(II)-requiring] activity; protein binding; iron ion binding; oxidoreductase activity

Biological Process: methionine salvage; sulfur amino acid metabolic process; polyamine metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with ADI1 elisa kit
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