• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ADIPOQ elisa kit :: Rat Adiponectin ELISA Kit

Scan QR to view Datasheet
Catalog # MBS8244709
Unit / Price
  96 Tests  /  $505 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Adiponectin (ADIPOQ), ELISA Kit

Popular Item
Also Known As

Rat Adiponectin ELISA Kit

Product Synonym Names
ACDC; ACRP30; APM1; GBP28; Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
244
3D Structure
ModBase 3D Structure for Q8K3R4
Species Reactivity
Specificity
The Rat Adiponectin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Adiponectin proteins within the range of 156 pg/ml - 10000 pg/ml.
Samples
Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates, Urine
Assay Type
Quantitative Sandwich
Sensitivity
<10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADIPOQ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADIPOQpurchase
MBS8244709 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adiponectin (ADIPOQ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADIPOQ. The ELISA analytical biochemical technique of the MBS8244709 kit is based on ADIPOQ antibody-ADIPOQ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADIPOQ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADIPOQ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADIPOQ elisa kit
Principle of the Assay: MBS Rat Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Adiponectin in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates, Urine. This assay employs an antibody specific for Rat Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
Applications Tested/Suitable for ADIPOQ elisa kit
Sandwich ELISA (EIA)

Typical Testing Data/Standard Curve (for reference only) of ADIPOQ elisa kit
ADIPOQ elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS8244709. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADIPOQ. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
26,410 Da
NCBI Official Full Name
30 kDa adipocyte complement-related protein (Adiponectin, C1Q and collagen domain containing)
UniProt Protein Name
30 kDa adipocyte complement-related protein
UniProt Synonym Protein Names
30 kDa adipocyte complement-related protein
Protein Family
UniProt Gene Name
Adipoq  [Similar Products]
UniProt Entry Name
Q8K3R4_RAT
UniProt Comments for ADIPOQ
adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.

Protein type: Hormone; Secreted; Secreted, signal peptide; Endoplasmic reticulum

Cellular Component: cell surface; collagen; endoplasmic reticulum; extracellular space; perinuclear region of cytoplasm; protein complex

Molecular Function: hormone activity; identical protein binding; protein homodimerization activity; receptor binding; sialic acid binding

Biological Process: adiponectin-mediated signaling pathway; brown fat cell differentiation; cellular response to insulin stimulus; circadian rhythm; fatty acid beta-oxidation; fatty acid oxidation; glucose homeostasis; glucose metabolic process; membrane depolarization; membrane hyperpolarization; negative regulation of blood pressure; negative regulation of cell migration; negative regulation of fat cell differentiation; negative regulation of gluconeogenesis; negative regulation of granulocyte differentiation; negative regulation of heterotypic cell-cell adhesion; negative regulation of hormone secretion; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of inflammatory response; negative regulation of low-density lipoprotein receptor biosynthetic process; negative regulation of macrophage differentiation; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of protein amino acid autophosphorylation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of synaptic transmission; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of blood pressure; positive regulation of cellular protein metabolic process; positive regulation of fatty acid metabolic process; positive regulation of glucose import; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 production; positive regulation of myeloid cell apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; positive regulation of signal transduction; protein homooligomerization; response to activity; response to drug; response to ethanol; response to glucocorticoid stimulus; response to glucose stimulus; response to hypoxia; response to nutrient; response to nutrient levels; response to sucrose stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000