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ADORA1 elisa kit :: Rat ADORA1 ELISA Kit

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Catalog # MBS2515905
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ADORA1, ELISA Kit

Popular Item
Also Known As

Rat ADORA1(Adenosine A1 Receptor)ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
326
OMIM
102775
3D Structure
ModBase 3D Structure for P30542
Species Reactivity
Specificity
This kit recognizes natural and recombinantRatADORA1. No significant cross-reactivity or interference between RatADORA1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADORA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADORA1purchase
MBS2515905 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADORA1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADORA1. The ELISA analytical biochemical technique of the MBS2515905 kit is based on ADORA1 antibody-ADORA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADORA1 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatADORA1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADORA1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADORA1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADORA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADORA1.You can calculate the concentration of ADORA1in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ADORA1 elisa kit
ADORA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2515905. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADORA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13,348 Da
NCBI Official Full Name
ADORA1, partial
NCBI Official Synonym Full Names
adenosine A1 receptor
NCBI Official Symbol
ADORA1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
adenosine receptor A1; adenosine A1 receptor variant 1
UniProt Protein Name
Adenosine receptor A1
Protein Family
UniProt Gene Name
ADORA1  [Similar Products]
UniProt Entry Name
AA1R_HUMAN
NCBI Summary for ADORA1
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ADORA1
ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: presynaptic membrane; asymmetric synapse; postsynaptic membrane; cell soma; integral to plasma membrane; basolateral plasma membrane; endoplasmic reticulum; postsynaptic density; plasma membrane; dendritic spine; presynaptic active zone; terminal button; axolemma

Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; adenosine receptor activity, G-protein coupled; purine nucleoside binding; phospholipase C activity; heterotrimeric G-protein binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; negative regulation of glutamate secretion; activation of MAPKK activity; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; neurological control of breathing; positive regulation of peptide secretion; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; cell-cell signaling; positive regulation of nucleoside transport; positive regulation of epidermal growth factor receptor activity; negative regulation of vasodilation; negative regulation of lipid catabolic process; negative regulation of blood pressure; negative regulation of synaptic transmission, GABAergic; protein targeting to membrane; detection of temperature stimulus involved in sensory perception of pain; inflammatory response; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of acute inflammatory response; negative regulation of synaptic transmission, glutamatergic; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; cognition; regulation of excitatory postsynaptic membrane potential; lipid catabolic process; positive regulation of blood pressure; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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