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ADORA2B elisa kit :: Mouse adenosine A2b receptor ELISA Kit

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Catalog # MBS903160
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

adenosine A2b receptor (ADORA2B), ELISA Kit

Popular Item
Also Known As

Mouse Adenosine receptor A2b, ADORA2B ELISA Kit

Product Synonym Names
Mouse Adenosine receptor A2b (ADORA2B) ELISA kit; ADORA2; ; adenosine A2b receptor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS903160 Testing Data
Sequence Length
332
3D Structure
ModBase 3D Structure for Q60614
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ADORA2B. No significant cross-reactivity or interference between Mouse ADORA2B and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell culture supernates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADORA2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADORA2Bpurchase
MBS903160 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenosine A2b receptor (ADORA2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADORA2B. The ELISA analytical biochemical technique of the MBS903160 kit is based on ADORA2B antibody-ADORA2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADORA2B elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADORA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ADORA2B elisa kit
ADORA2B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS903160. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADORA2B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,120 Da
NCBI Official Full Name
adenosine receptor A2b
NCBI Official Synonym Full Names
adenosine A2b receptor
NCBI Official Symbol
Adora2b  [Similar Products]
NCBI Official Synonym Symbols
A2b; A2BR; A2BAR; AA2BR; AI480866; AI605384
  [Similar Products]
NCBI Protein Information
adenosine receptor A2b; A2b, Rs
UniProt Protein Name
Adenosine receptor A2b
Protein Family
UniProt Gene Name
Adora2b  [Similar Products]
UniProt Entry Name
AA2BR_MOUSE
UniProt Comments for ADORA2B
ADORA2B: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; adenosine receptor activity, G-protein coupled

Biological Process: relaxation of vascular smooth muscle; negative regulation of collagen biosynthetic process; positive regulation of interleukin-6 production; positive regulation of guanylate cyclase activity; positive regulation of mast cell degranulation; positive regulation of vasodilation; signal transduction; G-protein signaling, adenylate cyclase activating pathway; regulation of angiogenesis; positive regulation of chemokine production; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; positive regulation of cGMP biosynthetic process; positive regulation of chronic inflammatory response to non-antigenic stimulus; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; cellular response to extracellular stimulus; positive regulation of catecholamine secretion; adenosine receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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