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ADRA2A elisa kit :: Canine Alpha-2A adrenergic receptor (ADRA2A) ELISA Kit

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Catalog # MBS7215126
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Alpha-2A adrenergic receptor (ADRA2A), ELISA Kit

Popular Item
Also Known As

Canine Alpha-2A adrenergic receptor (ADRA2A) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
104210
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ADRA2A. No significant cross-reactivity or interference between ADRA2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRA2A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADRA2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forADRA2Apurchase
MBS7215126 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-2A adrenergic receptor (ADRA2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADRA2A. The ELISA analytical biochemical technique of the MBS7215126 kit is based on ADRA2A antibody-ADRA2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADRA2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADRA2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ADRA2A elisa kit
Intended Uses: This ADRA2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADRA2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ADRA2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADRA2A antibody and an ADRA2A-HRP conjugate. The assay sample and buffer are incubated together with ADRA2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA2A concentration since ADRA2A from samples and ADRA2A-HRP conjugate compete for the anti-ADRA2A antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA2A from the sample, fewer sites are left to bind ADRA2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA2A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADRA2A elisa kit

Typical Standard Curve/Testing Data of ADRA2A elisa kit
ADRA2A elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7215126. Click to request current manual
NCBI/Uniprot data below describe general gene information for ADRA2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48,957 Da
NCBI Official Full Name
alpha-2A adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor alpha 2A
NCBI Official Symbol
ADRA2A  [Similar Products]
NCBI Official Synonym Symbols
ADRA2; ADRAR; ZNF32; ADRA2R; ALPHA2AAR
  [Similar Products]
NCBI Protein Information
alpha-2A adrenergic receptor; alpha-2A adrenoceptor; alpha-2AAR subtype C10; alpha-2A adrenoreceptor; adrenergic, alpha-2A-, receptor; alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type
UniProt Protein Name
Alpha-2A adrenergic receptor
UniProt Synonym Protein Names
Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR
UniProt Gene Name
ADRA2A  [Similar Products]
UniProt Synonym Gene Names
ADRA2R; ADRAR; Alpha-2A adrenoceptor; Alpha-2AAR  [Similar Products]
UniProt Entry Name
ADA2A_HUMAN
NCBI Summary for ADRA2A
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
UniProt Comments for ADRA2A
ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; synapse; receptor complex

Molecular Function: protein binding; protein homodimerization activity; alpha-2C adrenergic receptor binding; protein heterodimerization activity; thioesterase binding; alpha2-adrenergic receptor activity; norepinephrine binding; alpha-1B adrenergic receptor binding; epinephrine binding; protein kinase binding; heterotrimeric G-protein binding

Biological Process: fear response; negative regulation of calcium ion-dependent exocytosis; positive regulation of potassium ion transport; female pregnancy; thermoception; signal transduction; glucose homeostasis; positive regulation of vasodilation; positive regulation of membrane protein ectodomain proteolysis; Rho protein signal transduction; negative regulation of insulin secretion; intestinal absorption; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; positive regulation of cell proliferation; DNA replication; positive regulation of cytokine production; platelet activation; negative regulation of calcium ion transport; activation of protein kinase B; negative regulation of adenylate cyclase activity; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; regulation of vasoconstriction; Ras protein signal transduction; energy reserve metabolic process; activation of protein kinase activity; negative regulation of epinephrine secretion; cell motility; actin cytoskeleton organization and biogenesis; blood coagulation; regulation of insulin secretion; acute inflammatory response; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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