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aFGF recombinant protein :: Acidic Fibroblast Growth Factor Recombinant Protein

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Catalog # MBS203092
Unit / Price
  0.1 mg  /  $310 +1 FREE 8GB USB
  0.5 mg  /  $720 +1 FREE 8GB USB
SDS-PAGE
Product Name

Acidic Fibroblast Growth Factor (aFGF), Recombinant Protein

Popular Item
Also Known As

Acidic Fibroblast Growth Factor(aFGF), Human, Recombinant, E Coli

Product Synonym Names
FGF1; FGFA; Acidic Fibroblast Growth Factor (aFGF); HBGF-1; Acidic fibroblast growth factor; aFGF; Beta-endothelial cell growth factor; ECGF-beta; Heparin-binding growth factor 1; Acidic Fibroblast Growth Factor Acidic fibroblast growth factor; AFGF; Beta endothelial cell growth factor; ECGF; ECGF beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fibroblast growth factor 1; Fibroblast Growth Factor 1 Acidic; GLIO703; HBGF 1; HBGF1; Heparin binding growth factor 1; Heparin binding growth factor 1 precursor.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
155
Sequence
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Purity/Purification
> 95% by SDS - PAGE
Form/Format
Liquid. 20 mM Tris, pH 7.0, 100 mM NaCl
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Expression System
E Coli
Bioactivity
The ED50 for this effect is < 2.9 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 340,000 units/ml).
Assay
1. Cell Line: NIH-3T3 (Mus mosculus/ embryo)
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay Medium: serum free RPMI 1640
4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul)
5. Serum Free Starvation: 24hr with RPMI1640
6. Incubation Time: 24 hr (after sample treatment)
7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)
8. Detection method: Brdu assay
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of aFGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
aFGF recombinant protein
Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.
Product Categories/Family for aFGF recombinant protein
Applications Tested/Suitable for aFGF recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15.9kDa (141aa), confirmed by MALDI-TOF.
NCBI Official Full Name
fibroblast growth factor 1 isoform 1
NCBI Official Synonym Full Names
fibroblast growth factor 1 (acidic)
NCBI Official Symbol
NCBI Official Synonym Symbols
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
  [Similar Products]
NCBI Protein Information
fibroblast growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; heparin-binding growth factor 1
UniProt Protein Name
Fibroblast growth factor 1
UniProt Synonym Protein Names
Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1
UniProt Gene Name
UniProt Synonym Gene Names
FGFA; FGF-1; aFGF; ECGF; HBGF-1  [Similar Products]
UniProt Entry Name
FGF1_HUMAN
NCBI Summary for aFGF
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
UniProt Comments for aFGF
FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; nucleolus; extracellular region; cell cortex; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of cell division; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
Product References and Citations for aFGF recombinant protein
Jaye M. et al., (1986) Science. 233, 541-544.
Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129.
Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. u.S.A. 97, 49-54.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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