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anti-AFX antibody :: AFX Antibody

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Catalog # MBS858802
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

AFX, Antibody

Full Product Name

AFX (Phospho-Ser197) Antibody

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
300033
3D Structure
ModBase 3D Structure for P98177
Species Reactivity
Human, Mouse, Rat
Specificity
AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-AFX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AFX antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-AFX antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000

Immunohistochemistry (IHC) of anti-AFX antibody
P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (phospho-Ser197) antibody.
anti-AFX antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-AFX antibody
Western blot analysis of extracts from 293 cells using AFX (Ab-197) antibody (Line 1 and 2) and AFX (phospho-Ser197) antibody (Line 3 and 4).
anti-AFX antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AFX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,941 Da
NCBI Official Full Name
forkhead box protein O4 isoform 2
NCBI Official Synonym Full Names
forkhead box O4
NCBI Official Symbol
FOXO4  [Similar Products]
NCBI Official Synonym Symbols
AFX; AFX1; MLLT7
  [Similar Products]
NCBI Protein Information
forkhead box protein O4
UniProt Protein Name
Forkhead box protein O4
UniProt Synonym Protein Names
Fork head domain transcription factor AFX1
UniProt Gene Name
FOXO4  [Similar Products]
UniProt Synonym Gene Names
AFX; AFX1; MLLT7  [Similar Products]
NCBI Summary for AFX
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
UniProt Comments for AFX
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Product References and Citations for anti-AFX antibody
Di Maira G, et al. (2005) Cell Death Differ; 12(6): 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46. Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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