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AGC elisa kit :: Sheep AGC ELISA Kit

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Catalog # MBS2509778
Unit / Price
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  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $585 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

AGC, ELISA Kit

Full Product Name

Sheep AGC (Aggrecan) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q61282
Species Reactivity
Specificity
This kit recognizes natural and recombinant Sheep AGC. No significant cross-reactivity or interference between Sheep AGC and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AGC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AGCpurchase
MBS2509778 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AGC, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGC. The ELISA analytical biochemical technique of the MBS2509778 kit is based on AGC antibody-AGC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AGC elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep AGC concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AGC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AGC. You can calculate the concentration of AGC in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of AGC elisa kit
AGC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2509778. Click to request current manual
NCBI/Uniprot data below describe general gene information for AGC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
221,941 Da
NCBI Official Full Name
aggrecan core protein
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
NCBI Official Synonym Symbols
Agc; cmd; Agc1; Cspg1; b2b183Clo
  [Similar Products]
NCBI Protein Information
aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein
UniProt Protein Name
Aggrecan core protein
UniProt Synonym Protein Names
Cartilage-specific proteoglycan core protein; CSPCP
UniProt Gene Name
UniProt Synonym Gene Names
Agc; Agc1; CSPCP  [Similar Products]
UniProt Entry Name
PGCA_MOUSE
UniProt Comments for AGC
ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Cell adhesion

Cellular Component: proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: collagen fibril organization; central nervous system development; proteoglycan biosynthetic process; chondrocyte development; cell adhesion; negative regulation of cell migration; cartilage condensation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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