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AGPAT2 elisa kit :: Human 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) ELISA Kit

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Catalog # MBS2602770
Unit / Price
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  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) (AGPAT2), ELISA Kit

Popular Item
Full Product Name

Human 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, AGPAT2 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 608594
3D Structure
ModBase 3D Structure for O15120
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AGPAT2 concentration
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AGPAT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AGPAT2purchase
MBS2602770 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) (AGPAT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGPAT2. The ELISA analytical biochemical technique of the MBS2602770 kit is based on AGPAT2 antibody-AGPAT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGPAT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGPAT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AGPAT2 elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human AGPAT2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of AGPAT2 elisa kit
AGPAT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2602770. Click to request current manual
NCBI/Uniprot data below describe general gene information for AGPAT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,914 Da[Similar Products]
NCBI Official Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 2
NCBI Official Symbol
AGPAT2  [Similar Products]
NCBI Official Synonym Symbols
BSCL; BSCL1; LPAAB; 1-AGPAT2; LPAAT-beta
  [Similar Products]
NCBI Protein Information
1-acyl-sn-glycerol-3-phosphate acyltransferase beta; 1-AGPAT 2; 1-AGP acyltransferase 2; lysophosphatidic acid acyltransferase beta; lysophosphatidic acid acyltransferase-beta; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
UniProt Protein Name
1-acyl-sn-glycerol-3-phosphate acyltransferase beta
UniProt Synonym Protein Names
1-acylglycerol-3-phosphate O-acyltransferase 2; 1-AGP acyltransferase 2; 1-AGPAT 2; Lysophosphatidic acid acyltransferase beta; LPAAT-beta
UniProt Gene Name
AGPAT2  [Similar Products]
UniProt Synonym Gene Names
1-AGP acyltransferase 2; 1-AGPAT 2; LPAAT-beta  [Similar Products]
UniProt Entry Name
PLCB_HUMAN
NCBI Summary for AGPAT2
This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for AGPAT2
AGPAT2: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Defects in AGPAT2 are the cause of congenital generalized lipodystrophy type 1 (CGL1); also known as Berardinelli-Seip congenital lipodystrophy type 1 (BSCL1) or Berardinelli-Seip syndrome. CGL1 is an autosomal recessive disorder characterized by marked paucity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Transferase; Membrane protein, integral; Lipid Metabolism - ether lipid; EC 2.3.1.51

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: positive regulation of cytokine production; epidermis development; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; positive regulation of cytokine and chemokine mediated signaling pathway; CDP-diacylglycerol biosynthetic process

Disease: Lipodystrophy, Congenital Generalized, Type 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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