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Agrin elisa kit :: Sheep Agrin ELISA Kit

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Catalog # MBS742656
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Agrin elisa kit
Product Name

Agrin, ELISA Kit

Full Product Name

Sheep Agrin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
gene 254300
Species Reactivity
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Agrin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Agrinpurchase
MBS742656 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Agrin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Agrin. The ELISA analytical biochemical technique of the MBS742656 kit is based on Agrin antibody-Agrin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Agrin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Agrin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for Agrin elisa kit
NCBI/Uniprot data below describe general gene information for Agrin. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
216,733 Da
NCBI Official Full Name
agrin, partial
NCBI Official Synonym Full Names
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
agrin; agrin proteoglycan
UniProt Protein Name
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AGRIN; C90; C22  [Similar Products]
UniProt Entry Name
NCBI Summary for Agrin
This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. [provided by RefSeq, Aug 2011]
UniProt Comments for Agrin
AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.

Protein type: Cell development/differentiation; Cell adhesion; Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; cell surface; Golgi lumen; cytoplasm; extracellular region; plasma membrane; integral to membrane; basal lamina; synapse; cell junction

Molecular Function: heparan sulfate proteoglycan binding; protein binding; structural constituent of cytoskeleton; acetylcholine receptor regulator activity; laminin binding; calcium ion binding; sialic acid binding

Biological Process: positive regulation of filopodium formation; phototransduction, visible light; clustering of voltage-gated sodium channels; axon guidance; extracellular matrix organization and biogenesis; acetylcholine receptor signaling, muscarinic pathway; glycosaminoglycan metabolic process; neurotransmitter receptor metabolic process; pathogenesis; positive regulation of synaptic growth at neuromuscular junction; signal transduction; receptor clustering; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; plasma membrane organization and biogenesis; positive regulation of neuron apoptosis; carbohydrate metabolic process; synapse organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; retinoid metabolic process; neuromuscular junction development

Disease: Myasthenic Syndrome, Congenital, With Pre- And Postsynaptic Defects
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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