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AGT elisa kit :: Human angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit

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Catalog # MBS702389
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT), ELISA Kit

Popular Item
Full Product Name

Human angiotensinogen, aGT ELISA Kit

Product Synonym Names
Human angiotensinogen (aGT) ELISA Kit; ANHU; FLJ92595; FLJ97926; SERPINA8; alpha-1 antiproteinase; antitrypsin; angiotensin I; angiotensin II; angiotensinogen; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; angiotensinogen (serpin peptidase inhibitor; clade A; member 8)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS702389 Testing Data
OMIM
267430
3D Structure
ModBase 3D Structure for P01019
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human aGT. No significant cross-reactivity or interference between human aGT and analogues was observed.
Samples
Serum, plasma, urine, cell culture supernates
Assay Type
Sandwich
Detection Range
78 pg/ml -5000 pg/ml.
Sensitivity
The minimum detectable dose of human aGT is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AGT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AGTpurchase
MBS702389 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGT. The ELISA analytical biochemical technique of the MBS702389 kit is based on AGT antibody-AGT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AGT elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for aGT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any aGT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for aGT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of aGT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of AGT elisa kit
AGT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702389. Click to request current manual
NCBI/Uniprot data below describe general gene information for AGT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,154 Da
NCBI Official Full Name
angiotensinogen preproprotein
NCBI Official Synonym Full Names
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
NCBI Official Symbol
NCBI Official Synonym Symbols
ANHU; SERPINA8
  [Similar Products]
NCBI Protein Information
angiotensinogen; serpin A8; angiotensin I; angiotensin II; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor
UniProt Protein Name
Angiotensinogen
UniProt Synonym Protein Names
Serpin A8Cleaved into the following 8 chains:Angiotensin-1Alternative name(s):Angiotensin 1-10; Angiotensin I; Ang I
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SERPINA8; Ang I; Ang II; Ang III; Ang IV  [Similar Products]
UniProt Entry Name
ANGT_HUMAN
NCBI Summary for AGT
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008]
UniProt Comments for AGT
Function: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.3 PublicationsManual assertion based on experiment in:Ref.11
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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