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AGXT elisa kit :: Human alanine-glyoxylate aminotransferase ELISA Kit

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Catalog # MBS934004
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

alanine-glyoxylate aminotransferase (AGXT), ELISA Kit

Popular Item
Also Known As

Human Serine--pyruvate aminotransferase, AGXT ELISA Kit

Product Synonym Names
Human Serine--pyruvate aminotransferase (AGXT) ELISA kit; AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransfe; alanine-glyoxylate aminotransferase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS934004 Testing Data
Sequence Length
392
OMIM
604285
3D Structure
ModBase 3D Structure for P21549
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human AGXT. No significant cross-reactivity or interference between human AGXT and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich (Quantitative)
Detection Range
31.25 pg/ml-2000 pg/ml.
Sensitivity
7.81 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AGXT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAGXTpurchase
MBS934004 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alanine-glyoxylate aminotransferase (AGXT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGXT. The ELISA analytical biochemical technique of the MBS934004 kit is based on AGXT antibody-AGXT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGXT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGXT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AGXT elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGXT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGXT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGXT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGXT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of AGXT elisa kit
AGXT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS934004. Click to request current manual
NCBI/Uniprot data below describe general gene information for AGXT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,010 Da
NCBI Official Full Name
serine--pyruvate aminotransferase
NCBI Official Synonym Full Names
alanine-glyoxylate aminotransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
  [Similar Products]
NCBI Protein Information
serine--pyruvate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransferase; alanine--glyoxylate aminotransferase; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase
UniProt Protein Name
Serine--pyruvate aminotransferase
UniProt Synonym Protein Names
Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT
UniProt Gene Name
UniProt Synonym Gene Names
AGT1; SPAT; SPT; AGT  [Similar Products]
UniProt Entry Name
SPYA_HUMAN
NCBI Summary for AGXT
This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
UniProt Comments for AGXT
AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Motility/polarity/chemotaxis; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; EC 2.6.1.44

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: peroxisomal matrix; mitochondrial matrix; peroxisome

Molecular Function: amino acid binding; protein binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; pyridoxal phosphate binding; receptor binding

Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process

Disease: Hyperoxaluria, Primary, Type I
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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