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AHSG elisa kit :: Human alpha-2-HS-glycoprotein ELISA Kit

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Catalog # MBS701409 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

alpha-2-HS-glycoprotein (AHSG), ELISA Kit

Popular Item
Full Product Name

human Fetuin A ELISA Kit

Product Synonym Names
human Fetuin A ELISA Kit; A2HS; AHS; FETUA; HSGA; fetuin-A; alpha-2-HS-glycoprotein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS701409 Testing Data
OMIM
138680
3D Structure
ModBase 3D Structure for P02765
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human fetuin A. No significant cross-reactivity or interference between human fetuin A and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates, urine
Assay Type
Sandwich
Detection Range
15.6 ng/ml -1000 ng/ml.
Sensitivity
The minimum detectable dose of human fetuin A is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AHSG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AHSGpurchase
MBS701409 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-2-HS-glycoprotein (AHSG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AHSG. The ELISA analytical biochemical technique of the MBS701409 kit is based on AHSG antibody-AHSG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AHSG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AHSG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Proteases breakdown proteins but must be carefully regulated by endogenous inhibitors like Fetuin A. In a feedback regulatory loop this ABSG fetus glycoprotein is regulated by other factors in bone development. To understand this complex dance, quantitation by Fetuin ELISA is now possible thanks to MyBiosource and LSBio. We provide an excellent Fetuin alpha 2 ELISA kit with optimized antibody pairs, selective secondary antibodies and backed by our promise of value. Whether your assay sample types are cell culture media or human serum plasma, this sandwich format custom glycoprotein ELISA will accurately measure protein. ELISA kits come complete with all reagents, 96 tests and a Fetuin ABSG standard for comparison down to ng ml ranges. When you place your order for catalog number MBS701409, don't forget to check out our mouse Fetuin sandwich ELISA and polyclonal antibody.
Related Product Information for
AHSG elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for fetuin A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any fetuin A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for fetuin A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of fetuin A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of AHSG elisa kit
AHSG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701409. Click to request current manual
NCBI/Uniprot data below describe general gene information for AHSG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,325 Da
NCBI Official Full Name
alpha-2-HS-glycoprotein preproprotein
NCBI Official Synonym Full Names
alpha-2-HS-glycoprotein
NCBI Official Symbol
NCBI Official Synonym Symbols
AHS; A2HS; HSGA; FETUA
  [Similar Products]
NCBI Protein Information
alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein
UniProt Protein Name
Alpha-2-HS-glycoprotein
UniProt Synonym Protein Names
Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-ACleaved into the following 2 chains:Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FETUA; PRO2743  [Similar Products]
UniProt Entry Name
FETUA_HUMAN
NCBI Summary for AHSG
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]
UniProt Comments for AHSG
Function: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with AHSG elisa kit
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