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AICDA elisa kit :: Human activation-induced cytidine deaminase ELISA Kit

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Catalog # MBS940658
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Typical Testing Data/Standard Curve (for reference only)
Product Name

activation-induced cytidine deaminase (AICDA), ELISA Kit

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Full Product Name

Human Activation-induced cytidine deaminase, AICDA ELISA Kit

Product Synonym Names
Human Activation-induced cytidine deaminase (AICDA) ELISA kit; AID; ARP2; CDA2; HIGM2; integrated into Burkitt s lymphoma cell line Ramos; activation-induced cytidine deaminase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS940658 Testing Data
OMIM
gene 605258
3D Structure
ModBase 3D Structure for Q9GZX7
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human AICDA. No significant cross-reactivity or interference between human AICDA and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
34.4 pg/ml -2200 pg/ml.
Sensitivity
8.6 pg /ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AICDA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AICDApurchase
MBS940658 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the activation-induced cytidine deaminase (AICDA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AICDA. The ELISA analytical biochemical technique of the MBS940658 kit is based on AICDA antibody-AICDA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AICDA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AICDA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AICDA elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AICDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AICDA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AICDA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AICDA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of AICDA elisa kit
AICDA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS940658. Click to request current manual
NCBI/Uniprot data below describe general gene information for AICDA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,954 Da
NCBI Official Full Name
single-stranded DNA cytosine deaminase
NCBI Official Synonym Full Names
activation-induced cytidine deaminase
NCBI Official Symbol
AICDA  [Similar Products]
NCBI Official Synonym Symbols
AID; ARP2; CDA2; HIGM2; HEL-S-284
  [Similar Products]
NCBI Protein Information
single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos
UniProt Protein Name
Single-stranded DNA cytosine deaminase
UniProt Synonym Protein Names
Activation-induced cytidine deaminase; Cytidine aminohydrolase
UniProt Gene Name
AICDA  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
AICDA_HUMAN
NCBI Summary for AICDA
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]
UniProt Comments for AICDA
AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: Hydrolase; EC 3.5.4.38

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity

Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing

Disease: Immunodeficiency With Hyper-igm, Type 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withAICDA elisa kit
 Reference Product  PubMed Publications
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