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AKAP12 elisa kit :: Human A kinase (PRKA) anchor protein (gravin) 12 ELISA Kit

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Catalog # MBS723924
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
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AKAP12 elisa kit
Product Name

A kinase (PRKA) anchor protein (gravin) 12 (AKAP12), ELISA Kit

Full Product Name

Human A kinase (PRKA) anchor protein (gravin) 12 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
604698
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AKAP12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AKAP12purchase
MBS723924 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A kinase (PRKA) anchor protein (gravin) 12 (AKAP12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKAP12. The ELISA analytical biochemical technique of the MBS723924 kit is based on AKAP12 antibody-AKAP12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKAP12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKAP12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for AKAP12 elisa kit
NCBI/Uniprot data below describe general gene information for AKAP12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
180,990 Da
NCBI Official Full Name
A-kinase anchor protein 12 isoform 2
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 12
NCBI Official Symbol
AKAP12  [Similar Products]
NCBI Official Synonym Symbols
SSeCKS; AKAP250
  [Similar Products]
NCBI Protein Information
A-kinase anchor protein 12; AKAP 250; kinase scaffold protein gravin; A-kinase anchor protein, 250kDa; Src-Suppressed C Kinase Substrate; myasthenia gravis autoantigen gravin
UniProt Protein Name
A-kinase anchor protein 12
UniProt Synonym Protein Names
A-kinase anchor protein 250 kDa; AKAP 250; Gravin; Myasthenia gravis autoantigen
Protein Family
UniProt Gene Name
AKAP12  [Similar Products]
UniProt Synonym Gene Names
AKAP250; AKAP-12; AKAP 250  [Similar Products]
UniProt Entry Name
AKA12_HUMAN
NCBI Summary for AKAP12
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for AKAP12
AKAP12: a scaffold protein that contains 3 conserved PKA binding motifs. Suppresses podosome formation, inhibits RhoA and Cdc42 activity, and inhibits v-Src-induced oncogenic growth in NIH3T3. May inhibit Src-induced oncogenesis by reestablishing actin-based non-invasive cytoskeletal architecture.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6q24-q25

Cellular Component: cytoskeleton; focal adhesion; cytoplasm; plasma membrane; cell cortex

Molecular Function: protein binding; protein kinase A binding; adenylate cyclase binding

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cAMP biosynthetic process; protein targeting
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with AKAP12 elisa kit
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