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AKAP5 elisa kit :: Human A kinase anchor protein 5 (AKAP5) ELISA Kit

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Catalog # MBS7233081
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

A kinase anchor protein 5 (AKAP5), ELISA Kit

Full Product Name

Human A kinase anchor protein 5 (AKAP5) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
604688
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of KAP5. No significant cross-reactivity or interference between KAP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KAP5 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This KAP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KAP5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AKAP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AKAP5purchase
MBS7233081 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A kinase anchor protein 5 (AKAP5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKAP5. The ELISA analytical biochemical technique of the MBS7233081 kit is based on AKAP5 antibody-AKAP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKAP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKAP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AKAP5 elisa kit

Principle of the assay: KAP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KAP5 antibody and an KAP5-HRP conjugate. The assay sample and buffer are incubated together with KAP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KAP5 concentration since KAP5 from samples and KAP5-HRP conjugate compete for the anti-KAP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by KAP5 from the sample, fewer sites are left to bind KAP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KAP5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AKAP5 elisa kit

Typical Testing Data/Standard Curve (for reference only) of AKAP5 elisa kit
AKAP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7233081. Click to request current manual
NCBI/Uniprot data below describe general gene information for AKAP5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,088 Da
NCBI Official Full Name
A-kinase anchor protein 5
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 5
NCBI Official Symbol
AKAP5  [Similar Products]
NCBI Official Synonym Symbols
H21; AKAP75; AKAP79
  [Similar Products]
NCBI Protein Information
A-kinase anchor protein 5; AKAP 79; A-kinase anchor protein 79 kDa; A-kinase anchoring protein 75/79; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
UniProt Protein Name
A-kinase anchor protein 5
UniProt Synonym Protein Names
A-kinase anchor protein 79 kDa; AKAP 79; H21; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Protein Family
UniProt Gene Name
AKAP5  [Similar Products]
UniProt Synonym Gene Names
AKAP79; AKAP-5; AKAP 79  [Similar Products]
UniProt Entry Name
AKAP5_HUMAN
NCBI Summary for AKAP5
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]
UniProt Comments for AKAP5
AKAP5: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C. Interacts with ADCY8, and enhances its phosphorylation at lipid rafts. Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: plasma membrane; cytosol

Molecular Function: calmodulin binding; protein binding; protein kinase A binding; adenylate cyclase binding

Biological Process: synaptic transmission; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; signal transduction; regulation of insulin secretion; protein targeting
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with AKAP5 elisa kit
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